Phenol removal using immobilized biological laccase with bamboo charcoal

In order to improve the efficiency of laccase utilization and further develop its application of environmental governance, biological immobilization of laccase on bamboo charcoal for phenol removal was performed in this experiment. The effects of laccase immobilization, treatment time, concentration of phenol, carbon particle size, p H and solid/liquid ratio on the degradation of phenol were evaluated. The reaction time of 72 h, initial phenol concentration of 100 mg/L, biological carbon particle size of 10 mesh, p H of 4.5 and solid/liquid ratio of 1 ∶ 10 g/m L were optimal, which exhibited degradation ratio of phenol up to 100%.This study indicated an effective method for the treatment of phenol-containing pollution.