The bacteriostatic mechanism of phenyllactic acid with Listeria monocytogenes and Escherichia coli genome DNA

The interaction between phenyllactic acid (PLA) and Foodborne pathogenic bacteria genome (Listeria monocytogenes10403s and E.coli 44752) in vivo/vitro was studied by agarose gel electrophoresis and UV spectra.The results showed that there was no apparent interaction between PLA and the intracellular DNA of Listeria monocytogenes 10403s and E.coli 44752.The absorption UV spectra demonstrated that PLA could interact with bacterial genomic DNA in vitro in the manner of intercalation and groove binding.The binding constant K of PLA (16³2 mmol/L) was 9.16×10⁵M^-1for Listeria monocytogenes 10403s and the binding constant of PLA (2¹6 mmol/L) K=7.36×10⁵M^-1for E.coli 44752.The combination of PLA with DNA in vitro demonstrated that was static interaction and intercalation.