Simultaneous determination of 11 phenolic compounds in apple vinegar and sugarcane vinegar by HPLC-DAD

A new method for simultaneous determination of 11 phenolic compounds in fruit vinegar, such as different kinds of apple vinegar and sugarcane vinegar, by high performance liquid chromatography-diode array detector (HPLC-DAD) was established in this study.The phenolic compounds were separated by OMNI BOND with Hubble C₁₈column (250 mm×4.6 mm, 5μm) at 35℃.The mobile phase A was Me OH-HCOOH solution (100∶2, v/v) and the mobile phase B was 10 mmol/L K₂HPO₄-H₃PO₄ (200∶1, v/v) , eluted at a flow rate of 1.0 m L/min by a gradient elution program.The injection volume was20μL and the eluted components were monitored at 210⁴00 nm.Under the optimized condition, 11 phenolic compounds in different kinds of apple vinegar and sugarcane vinegar were separated and simultaneous determined, including coumarin, vanillin, chlorogenic acid, caffeic acid, ferulic acid, p-coumaric acid, apigenin, luteolin, cinnamic acid, kaempferol and formononetin, in which 10 phenolic components were found and determined in sugarcane vinegar.The recovery of the above method was 84.1%¹18.2%.The method is accurate, reliable, simple, feasible and suitable for determination of phenolic compounds in fruit vinegar.