Optimization of extraction technology and determination of antioxidant activity of polysaccharides from superfine powder of okra

The yield of polysaccharides in superfine powder that okra was prepared into was studied by ultrasonic-microwave assisted extraction method.The optimum process conditions were determined by response surface method.The antioxidant activity in vitro of polysaccharides extracted from superfine powder of okra was determined, including DPPH radical-scavenging, hydroxyl radical-scavenging,superoxide anion radical scavenging and reducing powers.The optimum extraction conditions were as follows:microwave time 2 min, ultrasonic time 14 min, and ultrasonic power 800 W. Under this optimized conditions, the experimental and predicted yield of polysaccharides was 27.68%±0.42%.The IC₅₀ for scavenging capacity of superfine powder of okra against DPPH·,O₂^-·,·OH and reducing power were 1.53,4.12,6.38 and 2.49 mg/mL respectively.