Preliminary study on extraction and antibacterial activity of total flavonoids in tangerine peel

Objective:To determine the best extraction conditions of the total flavonoids from tangerine peel,and preliminarily test of its antibacterial activity. Methods:The total flavonoids of tangerine peel(FTP)were extracted with ethanol through microwave-assisted method,and the Box-Behnken Design(BBD)experiment model was established according to the single factor experiments.And the results were validated by experimental verification.The FTP was purified by HP-20 macroporous resin. Then the antibacterial activity against E. enteritidis,Bacillus subtilis and Staphylococcus aureus was tested by filter paper method. Conclusion:The extraction results showed that yield of flavonoids was the highest 1.3468%±0.0142% when the concentration of ethanol was 60%,the microwave power was 619 W,the microwave time was 3 min,the extraction temperature was 62 ℃ and material to solvent ratio was 1:21 (g/mL),which had been verified by experiments. The deviation of the result to predicted value(1.3575%)was only 0.0107%,which indicated that the model and the actual fitted well.The antibacterial activity results showed that:FTP had different antibacterial activity against the tested bacterias,which was more effective against E. enteritidis and Staphylococcus aureus than the control drug tea polyphenols.