Effect of Pectinase Treatment on the Content of Soluble Sugar of Sweet Corncob

To increase the total sugar yield of sweet corncobs,the pectinase hydrolysis of sweet corncob was performed. Four factors of reactions were researched including enzyme addition,pH,temperature and time through single factor experiment. And the optimum extraction conditions of pectin hydrolysis were obtained by and response-assisted surface methods. The results showed that the optimum conditions for the hydrolysis of pectin as follows:Enzyme addition amount,temperature,pH and time was 88 U/g,60 ℃,5.0 and 90 min,respectively,at which the pectinase hydrolysis could reach 43.82%±0.02%. The soluble sugar content of sweet corn cobs before and after pectinase treatment was compared. The soluble sugar content before pectinase treatment was 53.16%±0.02%,the pectinase treatment was 62.89%±0.03%,and the soluble sugar content was increased by 9.73%.