Comparison and Optimization of Extraction Process of Polyphenols from Passiflora edulis Peel

With the Passiflora edulis peel as raw material,the optimum conditions of ethanol extraction and cellulose-assisted extraction of polyphenols in the peel were studied and the extraction effects were compared. Taking polyphenol yield as an index,the influence of various factors on the extraction of polyphenols was investigated by single factor test. The L₉(3⁴)orthogonal test was used to optimize the process conditions of cellulose extraction. The results showed that the optimal conditions of ethanol extraction were ethanol 60%,liquid to solid ratio 30:1 mL/g,extraction time 150 min,extraction temperature 40 ℃,and the polyphenol yield was(11.648±0.118) mg/g. And the optimal extraction conditions for enzymatic extraction were as follows:The amount of cellulase 25 mg/g,the liquid to material ratio 35:1 mL/g,the enzymolysis temperature 40 ℃,the enzymolysis time 60 min,pH=5,and the polyphenol yield was(15.096±0.948) mg/g. By comparing the maximum yield of polyphenols in two kinds of processing conditions,the extraction rate of polyphenols from cellulase assisted extraction method was 29.6% higher than that of ethanol extraction method. It proved that cellulase had better effect on the cell wall fragmentation of Passiflora edulis,and which would improve the extraction yield of polyphenols in peel.