Optimization of Preparation Process of Chitin from Penaeus Vannamei by Microwave Assisted Method

Chitin, from Penaeus vannamei in the Beibu Gulf of Guangxi, was prepared by microwave-assisted digestion. The preparation process was optimized by single factor experiment and orthogonal experiment.The results showed that the optimum deproteinized conditons were NaOH concentration 6%, microwave power 240 W, microwave time 7 min, material solution at 1:10 g/mL. and the optimum decalcification conditions were HCl concentration 5%, microwave power 160 W, microwave time 3 min, liquid ratio 1:40 g/mL.Under this condition,, deprotein rate reached 70.85%, decalcification rate was 91.26%, and the yield of chitin was 26.34% ±0.24%, which was better than that of the traditional acid base method 18.43% ±0.14%.