Protective Effects of Fibrinolytic Enzyme of Lyophyllum ulmarium on LPS-Induced Liver Injury in Rats

To observe the protective effects of fibrinolytic enzyme of Lyophyllum ulmarium (LUFE) in rats with liver injury induced by lipopolysaccharide (LPS). The rats were randomly divided into five groups:the normal control, model, positive control, and the low-and high-dose LUFE groups.Rats in the low-and high-dose LUFE groups were i.g given LUFE at dose of 200 and 400 mg/kg for a period of 2 weeks.Besides the control group, other four groups were i.p given lipopolysaccharide to induce inflammatory liver injury in rats.The positive control rats were i.p given 5 mg/kg dexamethasone before the model establishment.The morphological changes of liver were observed by light microscope.The levels of TNF-α, IL-6, IL-1β, ALT, AST and ALB in serum were measured.The expression of TLR4, p-TAK1 and NF-κB proteins was detected by Western blot. The results showed that LUFE could reduce the inflammatory damage in liver tissue, decrease the serum levels of TNF-α, IL-6, IL-1β, ALT, AST, increased the serum ALB, and down-regulate the expression of TLR4, p-TAK1 and p-NF-κB proteins in rats with liver injury.The above results indicated that LUFE had protective effects on the liver injury in rats.The effects may be related to the regulation of TLR4-NF-κB signaling pathway.