Study on Preparative Separation of Total Polyphenols from Sargassum thunbergii by Column Chromatography

In this paper,the analytical conditions for the adsorption of polyphenols on the seaweed by XDA-1B macroporous resin were studied. The results showed that the optimal conditions for static adsorption were as follows:temperature 30℃,polyphenol concentration in the sample 2.5 mg/mL,and XDA-1B macroporous adsorption resin was suitable for the separation and purification of polyphenols from Sargassum thunbergii. With the sample flow rate of 2.0 mL/min and the elution flow rate of 2.0 mL/min,the adsorption and desorption rate of the polyphenols on the XDA-1B macroporous resin was faster and the adsorption equilibrium was reached after 2 h,and Freundlich adsorption empirical formula is suitable for the adsorption curve simulation.