Effect of Ceramic Membrane Microfiltration Coupled with Macroporous Resin on Hydrolyzed Peptides of Tilapia Meat

The effect of inorganic ceramic membrane microfiltration and macroporous resin adsorption on the physical and chemical properties of the hydrolyzed peptides of tilapia were studied to provide reference for their development and utilization. Dynamic adsorption of tilapia hydrolyzed peptides by 50 nm ceramic membrane and AB-8 macroporous resin. The basic components were analyzed by national standards,and high molecular weight exclusion chromatography(HPSEC)was used to analyze the molecular weight distribution and amino acid analyz for determination of amino acid contents,reverse phase liquid phase analyze for amino acid hydrophobicity. Results showed that the microfiltration treatment of ceramic membrane had little effect on the amino acid composition of enzymatic polypeptides,and the proportion of 1~2,2~3,3~5,5~8 ku components decreased by 7.29%,17.13%,33.93% and 50.0% respectively after the microfiltration treatment of ceramic membrane,which indicated the interception effect of peptides,and the greater the molecular weight,the greater the interception ratio. The adsorption of AB-8 macroporous resin reduced the total amount of amino acids by 1.99%,the content of essential amino acids by 5.15%,and the contents of bitter amino acids and hydrophobic amino acids by 12.35% and 8.44%,respectively. And AB-8 macroporous resin was sensitive to the adsorption of 2~8 ku peptides,and less adsorption for free amino acids. RPLC results showed that the eluted fractions adsorbed by AB-8 macroporous resin contained more hydrophobic peptides. The dynamic adsorption of AB-8 macroporous resin could effectively improve the bitterness of the peptides digested by tilapia.