Screen and Optimization of the Methods for Removing Protein from Crude Polysaccharide in Ginkgo Biloba Leaves

In this paper,the effects of sevage method,TCA method,isoelectric point method and enzymatic method on the removal of ginkgo biloba leaves polysaccharide protein were compared with the indicator of the protein clearance and loss rate of polysaccharide.Results showed that both enzymatic and isoelectric methods were good methods for removing proteins. And the best optimized steps of enzymatic-isoelectric point method were as follows:The enzyme addition amount of 0.90%(calculated as substrate),the enzymatic hydrolysis time of 1.1 h,the enzymatic hydrolysis temperature of 46 ℃,and the enzymatic hydrolysis pH of 4.7. Under the conditions,the protein loss rate was 92.73%±0.13%,and the polysaccharide loss rate was 9.28%±0.12%. This method had a higher protein rate and loss rate of polysaccharide was low. It would provide an experimental basis for the utilization of polysaccharides in the food field.