Determination of 9 Biogenic Amines in Pork by Using Ultra-high Performance Liquid Chromatography

Objective:To develop and optimized ultra high pressure liquid chromatography(UPLC)methods for the quantitative determination of tryptamine,β-phenylethylamine,putrescine,cadaverive,histamine,octopamine,tyramine,spermidine and spermine in pork. Methods:The samples were extracted with 5% TCA and derived with dansyl chloride. (2.1×100 mm,1.7 μm)C₁₈ column was used. The mobile phase consisted of acetonitrile-10 mmol/L ammonium acetate solution at the flow rate of 0.3 mL/min.The detection wavelength was at 245 nm and the column temperature was 35 ℃.The injection volume was 3.0 μL.Results:The linear range of 9 biogenic amines was from 5.0 to 100.0 μg/mL(r≥0.999). The limits of detection were in the range of 0.028~0.29 mg/kg.The relative standard deviation(RSD)of precision was lower than 3.72%.The average recovery was 70.70%~111.80%. Conclusions:The method was simple,accurate,sensitive,reproducible and short peak time. It would have a nice application foreground in a quick,accurate and batch quantity analysis methods of biogenic amines in pork.