Optimization of Extraction of Free Amino Acids from the Meat of Female and Male Chinese Mitten Crab(Eriocheir sinensis)

This study aimed to obtain the optimum conditions for the extraction of free amino acids from the meat of Chinese Mitten Crab(Eriocheir sinensis),the ultrasonic time,ultrasonic temperature,ultrasonic power,extraction times and liquid-to-liquid ratio were studied by the orthogonal test. The results showed that,the effects of factors on the extraction yield of amino acids were in the descending order of solid-to-liquid ratio>number of extraction cycles>ultrasonic time>temperature. The optimum level of male crab was determined by ultrasonic power 200 W,extraction temperature 40℃,ultrasonic ratio extraction 1:30 (g/mL)for 7 min,extraction times was 3.Under these conditions,the actual extraction yield of free amino acids for male crab was(26.227±0.244) mg/g. The optimum level of female crab was determined by ultrasonic power 200 W,extraction temperature 20℃,ultrasonic ratio extraction 1:40 (g/mL)for 5 min,extraction times was 2.Under these conditions,the actual extraction yield of free amino acids for female crab was(25.101±0.135) mg/g.