Research on Large-scaled Preparation of High Purity Anthocyanins from Mulberry and Raspberry by Medium Pressure Rapid Separation System

In order to quickly obtain a large number of different structural anthocyanins,in this paper,two crude anthocyanins were separated from rich-anthocyanins fruits namely mulberry and raspberry by macroporous adsorption resin AB-8,and high purity mulberry and raspberry anthocyanins were isolated by medium pressure rapid separation system. The specific preparation conditions were as follows:flash C₁₈(80 g,20~35 μm,100A)as the preparation column,two in series,using 2% of aqueous formic acid as phase A(v/v),and methanol as phase B with the gradient program of:0~2 min,20% B;2~22 min,20%~30% B;22~32 min,30%~40% B,the flow rate was 30 mL/min,and sample loading was 300 mg. The separation and purification three different structures of anthocyanins were achieved. The purity of cyanidin 3-glucoside and cyanidin 3-rutinoside,which were purified from mulberry fruits,were about 95 and 41%,respectively. Meanwhile,the purity of cyanidin 3-sophoroside,and cyanidin 3-glucoside was about 60% and 75%,respectively. Among them,30 mg of cyanidin 3-glucoside from mulberry fruits could be obtained for one run in a 32 min gradient program,and the purity was 95%,which could meet the requirements of the standard.