Extraction,Component Analysis and Antioxidant Activity of Rana chensinensis Egg Oil

The egg oil of Rana chensinensis was extracted by supercritical CO₂ extraction from the eggs of Rana chensinensis,the component content and antioxidant activity of the Rana chensinensis egg oil were researched.The components and the contents of Rana chensinensis egg oil were analyzed and detected used the method of gas chromatography-mass spectrometry(GC-MS). DPPH radical scavenging ability test,hydroxyl radical scavenging test and superoxide anion free radical scavenging test were carried out for determining the antioxdant capactity of Rana chensinensis egg oil.The optimum extraction conditions were determined by single factor test and orthogonal test were as follows:Extraction pressure 35 MPa,extraction temperature 60 ℃,CO₂ flow rate 10 L/h and extraction time 150 min. The extraction rate of Rana chensinensis egg oil was 34.26%.The results of GC-MS showed that there were 34 fatty acids in the frog egg oil,the relative contents of saturated fatty acids and unsaturated fatty acids were 31.95% and 68.05%,respectively.The results of in vitro antioxidant test suggested that the IC₅₀ value of Rana esculenta egg oil to DPPH free radical was 0.897 mg/mL,the IC₅₀ value for hydroxyl radicals was 1.392 mg/mL,and the IC₅₀value of superoxide anion was 1.687 mg/mL.The frog egg oil had effective activity in vitro antioxidant experiment and showed certain development and utilization value in natural antioxidants and health foods.