Identification of Sheep-derived Components and Their Content in Cold Fresh Meat Products Based on Fluorescent Quantitative PCR

In order to establish a method for quickly and accurately identifying sheep-derived components in sheep products and quantifying the content of sheep components,specific primers were designed with sheep mitochondrial cytochrome b gene as the target gene.The eukaryotic ribosomal 16S rDNA was selected as the internal reference gene,and the real-time fluorescence relative quantification method was used.The logarithmic value of the percentage of mutton mass had a good linear relationship with the corresponding cycle threshold difference ΔCt.The standard curve regression formula was y=-3.4057x-0.3112,R²=0.9916,and the amplification efficiency reached 96.62%. In this test,the detection limit of sheep-derived DNA could reach 16 pg/μL,and the recovery rate was between 93.07% and 106.20%. Taking 8 commercial mutton products for testing,3 of them were below 50%.The real-time fluorescence PCR detection method established in this experiment was convenient to operate with strong specificity,high sensitivity,and reliable data,which provided a reference method for the detection of sheep-derived components in meat products.