Optimization of Solid Phase Acid Hydrolysis of Polysaccharide from Abalone Viscera by Response Surface Methodology

In order to study relationship between the solid-phase acid(732-cation exchange resin)hydrolysis conditions of polysaccharide from abalone viscera and the hydrolytic rate of polysaccharide and the molecular structure of its degradation products. Firstly,the effects of resin dosage,hydrolysis time and hydrolysis temperature on the hydrolysis rate of abalone viscera polysaccharide were determined by single factor experiments. Then response surface methodology was used to optimize the hydrolysis conditions of abalone viscera polysaccharide.Finally,the abalone viscera oligosaccharide mixture was separated and purified by Sephadex G-25 gel column,and the structure of oligosaccharide was determined by infrared spectroscopy.The results showed that the degree of influence on the hydrolysis rate of polysaccharide was hydrolysis temperature,hydrolysis time and resin dosage in turn,and the optimal hydrolysis conditions were as follows:Resin dosage 40 g,hydrolysis time 3 h,hydrolysis temperature 70 ℃. Under three conditions of parallel verification test,the hydrolysis rate of abalone viscera polysaccharide was 80.69%,which was close to and slightly higher than the predicted value,indicating that it was feasible to optimize the hydrolysis conditions of abalone viscera polysaccharide by response surface methodology. Purification by gel chromatography showed that there were two components in the abalone viscera oligosaccharide mixture,and the first component accounted for the majority.Infrared spectroscopy analysis showed that the abalone viscera oligosaccharides were α-type sugars and contained pyran ring.This study would improve economic efficiency of abalone viscera processing,and provide reference for its efficient utilization.