Optimization of Extraction Process and Component Analysis of Anthocyanins from Grape Pomace in Aqueous Two-phase Extraction with Genetic Algorithm

The anthocyanins were extracted from grape pomace by aqueous two-phase extraction. Genetic algorithm was employed to optimize the extraction process of anthocyanins from grape pomace based on single factor experiment. HPLC-MS was used to identify the anthocyanins components of the extracts from grape pomace obtained under the optimal combination of process parameters. The optimum extracting parameters to achieve the highest the yield of anthocyanins of(3.05±0.07) mg/g from grape pomace by aqueous two-phase method was obtained under an ethanol concentration of 40%,ammonium sulfate mass fraction of 26%,pH of 3.0,solid-to-liquid ratio of 1:38 g/mL. Under these conditions,the yield of anthocyanims was(3.05±0.07) mg/g.The anthocyanins extracts from grape pomace mainly contained two anthocyanins components in terms of delphinium-3-glucoside and cyanidin-3-glucoside,and their purities were 90.16% and 92.41%,respectively. The results of natural anthocyanins extraction would provide a new way and basis for further development of functional food.