Screening,Identification and Biological Characteristics of Ferulic Acid Esterase Strain in Luzhou-flavor Liquor Brewing Process

In this study,a ferulic esterase producing bacterium was selected from Daqu,pit mud and distilled grains of Luzhou-flavor liquor,and its physiological and biochemical characteristics were studied. The target strains were isolated and purified by plate lineation,and then the isolated strains were identified according to morphological characteristics and homologous sequence analysis of 16S rRNA. The results showed that Kosakonia cowani was the strain which produced ferulic acid esterase,and the ferulic acid esterase activity was 25.85 U/L. The biological characteristics of this strain were as follows:The biomass of the strain reached its maximum at 16 h,the optimal growth temperature was 32 ℃,the initial pH of the optimal growth medium was 5.The strain could grow normally in the range of 1%~9% NaCl and 1%~4% alcohol.