Effects of Neohesperidin on the Differentiation of Adipocyte and the Underlying Mechanism in 3T3-L1 Preadipocytes

GUO Lixia; KONG Shuzhen; YIN Zhongyi; ZHENG Xuxu

doi:10.13386/j.issn1002-0306.20201001641

Volume 42 Issue 12

Jun. 2021

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Article Navigation > Science and Technology of Food Industry > 2021 > 42(12): 125-132

GUO Lixia, KONG Shuzhen, YIN Zhongyi, et al. Effects of Neohesperidin on the Differentiation of Adipocyte and the Underlying Mechanism in 3T3-L1 Preadipocytes [J]. Science and Technology of Food Industry, 2021, 42(12): 125−132. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.20201001641

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Effects of Neohesperidin on the Differentiation of Adipocyte and the Underlying Mechanism in 3T3-L1 Preadipocytes

doi: 10.13386/j.issn1002-0306.20201001641

Key Laboratory of Natural Medicine Research of Chongqing Education Commission, Environmental and Resources Institute, Chongqing Technology and Business University, Chongqing 400067, China

Received Date: 2020-10-21
Available Online: 2021-05-26
Publish Date: 2021-06-03

Abstract

Abstract

This investigation aimed to identify the effects of neohesperidin on the differentiation of adipocyte and the underlying mechanism in 3T3-L1 preadipocytes. The cell viabilities of neohesperidin were detected in 3T3-L1 cells by MTS assay. The intracellular accumulation of lipids was visualized with Oil-red O staining and spectrophotometry analysis. The mRNA of CCAAT/enhancer binding protein α(C/EBPα) and peroxisome proliferators-activated receptorγ(PPARγ), adipogenic-specific genes during adipocyte differentiation, were measured by RT-PCR. Glycogen synthase kinase3β (GSK3β), glycogen synthase (GS) andprotein phosphorylation, the Akt signaling pathway, were analysed by immunoblotting. To confirm this GSK3β effect, 3T3-L1 cells were incubated with neohesperidin and GSK3β inhibitor (LiCl), and the intracellular accumulation of lipids and the level of adipogenic-specific protein were measured.The results showed that, neohesperidin significantly inhibited adipocyte differentiation and intracellular triglyceride formation (P<0.01), activated Akt signaling pathway, and promoted p-Akt and p-gsk3β, significantly inhibited C/EBPα, PPARγ, the expression of AP2 mRNA and protein (P<0.01). These effects were partly reversed by inhibition of GSK3β activity by LiCl. In summary, neohesperidin suppresses adipocyte differentiation via the Akt/GSK3β signaling pathway in 3T3-L1 preadipocytes.
- neohesperidin,
- Akt/GSK3β signaling pathway,
- adipogenic differentiation

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References(30)

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