Physicochemical Properties,Antioxidant and Antibacterial Capacities of Non-starch Polysaccharide from Dolichos lablab L.
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摘要: 以白扁豆为原料,提取得到白扁豆非淀粉多糖(Non-starch Polysaccharide from Dolichos lablab L,NS-DLP),并研究其理化性质、抗氧化活性及抑菌性能。通过热水浸提法制备NS-DLP,采用红外光谱、离子色谱、扫描电镜等技术分析NS-DLP的理化性质和表观形貌;通过OH自由基清除能力和DPPH自由基清除能力研究NS-DLP的体外抗氧化能力;采用抑菌圈法研究NS-DLP的抑菌性能。结果显示,NS-DLP具有吡喃环结构,平均分子质量2.3×105 Da,其单糖由葡萄糖、鼠李糖、半乳糖醛酸、半乳糖、木糖、阿拉伯糖组成,质量百分比(%)为23.23:6.2:5.09:2.76:2.4:0.48,电镜扫描结果显示该多糖表面微观形貌为片状。抗氧化实验表明NS-DLP对OH自由基和DPPH自由基具有一定清除作用,清除能力与浓度呈现明显的量效关系。当NS-DLP浓度达到6.4 mg/mL时,对OH自由基和DPPH的清除率分别为44.43%±2.41%、21.20%±1.33%。抑菌实验结果显示NS-DLP在李斯特菌中体现出最明显的抑菌圈,抑菌圈直径达到11.23 mm,在大肠杆菌中次之,抑菌圈直径为10.23 mm,表明NS-DLP对李斯特菌和大肠杆菌有一定的抑制能力。综上,NS-DLP具有一定的抗氧化性和抑菌性能,可为白扁豆的深加工和进一步研究开发奠定理论基础。Abstract: In order to explore the characterization of non-starch polysaccharide from Dolichos lablab L. (NS-DLP)referring to its physicochemical properties,antioxidant activities and antibacterial capacities. In this work,hot water extraction was employed to extracted NS-DLP,and its physicochemical properties and apparent structure were determined by high performance anion exchange chromatography,Fourier transform infrared spectroscopy,and scanning electron microscope,respectively. Furthermore,the antioxidant abilities of NS-DLP were evaluated by DPPH free radical scavenging and hydroxyl free radical scavenging activities. Meanwhile,the antimicrobial activities of NS-DLP were further investigated. The results showed that pyran ring was existed in NS-DLP which molecular weights were 2.3×105 Da. Moreover,the analysis of monosaccharide composition showed that NS-DLP was composed of glucose,rhamnose,galacturonic acid,galactose,xylose and arabinose,along with the mass ratio(%)of 23.23:6.2:5.09:2.76:2.4:0.48,and sheet structures were found in NS-DLP under the scanning electron microscope. Besides,antioxidant analysis indicated that NS-DLP presented hydroxyl radical and DPPH free radical scavenging activities in a dose-dependent manner up to 6.4 mg/mL,in association with their scavenging capacities of 44.43%±2.41% and 21.20%±1.33% correspondingly. Subsequently,the data of bacteriostasis test demonstrated that NS-DLP diaplayed certain inhibitory activities by inhibiting Listeria and Escherichia coli. The diameter of bacteriostatic circle of Listeria was 11.23 mm,and the diameter of bacteriostatic circle of Escherichia coli was 10.23 mm. In summary,NS-DLP presented certain antioxidant and bacteriostatic capacities,which could provide a theoretical foundation for extend processing and developing for the application of white lentil.
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