Abstract: In order to explore the characterization of non-starch polysaccharide from Dolichos lablab L. (NS-DLP)referring to its physicochemical properties,antioxidant activities and antibacterial capacities. In this work,hot water extraction was employed to extracted NS-DLP,and its physicochemical properties and apparent structure were determined by high performance anion exchange chromatography,Fourier transform infrared spectroscopy,and scanning electron microscope,respectively. Furthermore,the antioxidant abilities of NS-DLP were evaluated by DPPH free radical scavenging and hydroxyl free radical scavenging activities. Meanwhile,the antimicrobial activities of NS-DLP were further investigated. The results showed that pyran ring was existed in NS-DLP which molecular weights were 2.3×10⁵ Da. Moreover,the analysis of monosaccharide composition showed that NS-DLP was composed of glucose,rhamnose,galacturonic acid,galactose,xylose and arabinose,along with the mass ratio(%)of 23.23:6.2:5.09:2.76:2.4:0.48,and sheet structures were found in NS-DLP under the scanning electron microscope. Besides,antioxidant analysis indicated that NS-DLP presented hydroxyl radical and DPPH free radical scavenging activities in a dose-dependent manner up to 6.4 mg/mL,in association with their scavenging capacities of 44.43%±2.41% and 21.20%±1.33% correspondingly. Subsequently,the data of bacteriostasis test demonstrated that NS-DLP diaplayed certain inhibitory activities by inhibiting Listeria and Escherichia coli. The diameter of bacteriostatic circle of Listeria was 11.23 mm,and the diameter of bacteriostatic circle of Escherichia coli was 10.23 mm. In summary,NS-DLP presented certain antioxidant and bacteriostatic capacities,which could provide a theoretical foundation for extend processing and developing for the application of white lentil.