Abstract: Object:To study the activation effect of phenylethanol glycosides extracted from Cistanche tubulosa on macrophages,and to screen for a prescription,to investigate its immune-enhancing effect on normal mice. Methods:Studied on the role of phenylethanol glycosides on macrophage RAW264.7 activation and TNF-α,IL-6 production by the macrophage activation experiments.Compared the effects of angelica and astragalus on the activation of macrophages of phenylethanol glycoside extract.To evaluate the immune-enhancing function of tablets,which were prepared based on the screening test,by the ConA induced mice spleen lymphocyte transformation test,delayed-type hypersensitivity test,antibody-forming cell test,mice carbon clearance test and macrophage devour chicken erythrocyte test. Results:The results showed that 75,100,125 g/L groups could improve the activity of RAW264.7 cells,improve the level of NO,iNOS,TNF-α and IL-6,and 100 g/L group had the best effects(P<0.01). And the adding of angelica and astragalus can improve the activation function of phenylethanol glycoside on macrophages. The animal experiment of tables showed that 0.4 and 1.2 g/kg·bw/d groups could promote lymphocyte proliferation(P<0.05),1.2 g/kg·bw/d group could promote cellular immune response(P<0.05),0.4 g/kg·bw/d group could promote antibody production(P<0.05),1.2 g/kg·bw/d group could improve the ability of carbon clearance and macrophage phagocytosis(P<0.05). Conclusion:Phenylethanol glycosides extracted from Cistanche tubulosa may be to stimulate NO synthesis through induce iNOS expression,and to stimulate TNF-α and IL-6 releases,thus activate the immunomodulation of macrophage.The compound tablet prepared with phenylethanol glycosides angelica and astragalus can enhance the immunity of normal mice by regulating cellular immunity and monocyte-macrophage function,and exert positive effects on humoral immunity.