Abstract: Objective: To clarify the distribution patterns and make full use of Chinese wild resources Vitis davidii fruit in different tissues. Methods: Ultrasonic-assisted extraction and HPLC-MS/MS techniques were used for the determination of taxifolin. The response surface analysis was used to optimize the extraction process of taxifolin and the single factor test. Results: The taxifolin content in fruit peel was 3.63±0.18mg/kg, significantly (P<0.05) higher than that in seed (1.74±0.13mg/kg) and pulp (0.35±0.02mg/kg). The extraction efficiency was the highest when the ethanol concentration was 50%, and the extraction time was proportional to the extraction amount within 4 h. The dynamic equilibrium reached between extraction time and extraction amount after 4 h. The extraction amount reached the peak at 50 ℃. The optimal extraction conditions were finally determined with ethanol 49%, soaking temperature 30 ℃, soaking time 1 h, ultrasonic power 100 W, ultrasonic time 0.5 h, extraction time 4 h and extraction temperature 51 ℃. With the optimal conditions, the taxifolin extraction amount was up to 3.587 mg/kg. Conclusion: Chinese wild Vitis davidii fruit was rich in taxifolin, this study would provide a theoretical basis for the processing and utilization of Vitis davidii.