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中国精品科技期刊2020
杨琳,赵姗彦,杨帆,等. 五指毛桃黄酮固体饮料制备工艺优化[J]. 食品工业科技,2025,46(10):212−219. doi: 10.13386/j.issn1002-0306.2024060160.
引用本文: 杨琳,赵姗彦,杨帆,等. 五指毛桃黄酮固体饮料制备工艺优化[J]. 食品工业科技,2025,46(10):212−219. doi: 10.13386/j.issn1002-0306.2024060160.
YANG Lin, ZHAO Shanyan, YANG Fan, et al. Optimization of the Preparation Process of Flavonoid Solid Beverage from Ficus hirta Vahl. DOI: 10.13386/j.issn1002-0306.2024060160
Citation: YANG Lin, ZHAO Shanyan, YANG Fan, et al. Optimization of the Preparation Process of Flavonoid Solid Beverage from Ficus hirta Vahl. DOI: 10.13386/j.issn1002-0306.2024060160

五指毛桃黄酮固体饮料制备工艺优化

Optimization of the Preparation Process of Flavonoid Solid Beverage from Ficus hirta Vahl.

  • 摘要: 为研究五指毛桃黄酮固体饮料制备工艺,以乙醇为溶剂,超声辅助提取五指毛桃中黄酮类化合物,通过单因素实验和响应面试验优化五指毛桃黄酮提取工艺,并采用高效液相色谱质谱联用技术(HPLC-MS/MS)测定提取物中黄酮种类,制备五指毛桃黄酮固体饮料。实验结果表明:五指毛桃黄酮超声提取的最佳条件为:乙醇浓度72%,液固比40:1(mL/g),超声时间32 min,此工艺条件下测得总黄酮提取量为9.016 mg/g。纯化五指毛桃黄酮,共检测到32种黄酮类物质,包括黄酮组24种,异黄酮组8种。五指毛桃黄酮纯化前后,DPPH自由基和ABTS+自由基清除率IC50分别由0.678 mg/mL和0.501 mg/mL降低至0.179 mg/mL和0.191 mg/mL。五指毛桃黄酮浓度在1.0 mg/mL时,还原力由纯化前的0.066提升至纯化后的0.228。添加20%白砂糖,0.06%柠檬酸,2.5%麦芽糊精,制备的五指毛桃固体饮料感官评价得分最高,为五指毛桃的功能性产品开发和利用提供指导。

     

    Abstract: In order to study the preparation process of Ficus hirta flavone solid beverage, ethanol was used as a solvent and ultrasonication was assisted to extract flavone. The extraction process of flavone was optimised by single factor experiment and response surface experiments, and the flavone species in the extract were determined by high performance liquid chromatography mass spectrometry (HPLC-MS/MS). Finally, the solid beverage of Ficus hirta flavone was prepared. The experimental results showed that the optimal conditions for the ultrasonic extraction of Ficus hirta flavone were as follows: Ethanol concentration of 72%, liquid-solid ratio of 40:1 (mL/g), and ultrasonic time of 32 min, and the total flavone extracted under this process condition was measured to be 9.016 mg/g. A total of 32 flavones were detected in the purified Ficus hirta, including 24 flavonoids and 8 isoflavonoids. Before and after the purification of Ficus hirta flavone, the DPPH and ABTS+ scavenging rate IC50 decreased from 0.678 mg/mL and 0.501 mg/mL to 0.179 mg/mL and 0.191 mg/mL, respectively. The reducing power of Ficus hirta at a concentration of 1.0 mg/mL was increased from 0.066 before the purification to 0.228 after the purification. The Ficus hirta solid beverage prepared by adding 20% sugar, 0.06% citric acid, and 2.5% maltodextrin had the highest sensory evaluation score, which provided guidance for the development and utilization of functional products of Ficus hirta.

     

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