• 中国科技期刊卓越行动计划项目资助期刊
  • 中国精品科技期刊
  • EI
  • Scopus
  • CAB Abstracts
  • Global Health
  • 北大核心期刊
  • DOAJ
  • EBSCO
  • 中国核心学术期刊RCCSE A+
  • 中国科技核心期刊CSTPCD
  • JST China
  • FSTA
  • 中国农林核心期刊
  • 中国开放获取期刊数据库COAJ
  • CA
  • WJCI
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
中国精品科技期刊2020
孙永进,王泓杰,黄静,等. 羧化壳聚糖-果胶基纳米银的制备、结构表征及抗氧化活性分析[J]. 食品工业科技,2025,46(12):91−100. doi: 10.13386/j.issn1002-0306.2024070043.
引用本文: 孙永进,王泓杰,黄静,等. 羧化壳聚糖-果胶基纳米银的制备、结构表征及抗氧化活性分析[J]. 食品工业科技,2025,46(12):91−100. doi: 10.13386/j.issn1002-0306.2024070043.
SUN Yongjin, WANG Hongjie, HUANG Jing, et al. Preparation, Structural Characterization and Antioxidant Activity of Carboxylated Chitosan-Pectin-based Silver Nanoparticles[J]. Science and Technology of Food Industry, 2025, 46(12): 91−100. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024070043.
Citation: SUN Yongjin, WANG Hongjie, HUANG Jing, et al. Preparation, Structural Characterization and Antioxidant Activity of Carboxylated Chitosan-Pectin-based Silver Nanoparticles[J]. Science and Technology of Food Industry, 2025, 46(12): 91−100. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024070043.

羧化壳聚糖-果胶基纳米银的制备、结构表征及抗氧化活性分析

Preparation, Structural Characterization and Antioxidant Activity of Carboxylated Chitosan-Pectin-based Silver Nanoparticles

  • 摘要: 纳米银(AgNPs)作为一种安全、稳定、高效的新型抗氧化剂,在食品包装领域和医学领域具有良好的应用前景。本文以羧化壳聚糖(Carboxylated chitosan)和果胶(Pectin)为原材料,采用微波辅助法合成羧化壳聚糖-果胶基纳米银(CP-AgNPs)和果胶纳米银(P-AgNPs)。通过单因素实验获得AgNPs的最优合成条件,应用傅里叶红外光谱法(FTIR)、扫描电镜(SEM)分析、透射电镜(TEM)分析、Zeta电位分析、X-射线衍射和热重分析(TGA)等对AgNPs进行结构表征,并对其抗氧化活性进行评价。结果表明:优化后的合成条件为微波功率480 W、微波温度60 ℃、微波时间4 min、硝酸银浓度0.7 mol/L。用最优合成条件制得CP-AgNPs和P-AgNPs,410 nm处有AgNPs的特征吸收峰,SEM表明CP-AgNPs和P-AgNPs呈球形,粒径分别为33.18 nm和19.44 nm,均为面心立方结构晶体,CP-AgNPs相较于P-AgNPs具有更好的稳定性。活性研究表明CP-AgNPs具有较好的抗氧化活性,对DPPH自由基、羟基自由基、ABTS+自由基清除率最高分别为85.30%、63.35%、54.10%。采用微波辅助法可合成一种稳定、无污染、高效的新型纳米银抗氧化剂,并在食品加工、医学等领域具有重要的应用价值。

     

    Abstract: Silver nanoparticles (AgNPs) were demonstrated to have considerable promise in practical applications, particularly in the domains of food packaging and medicine. The development of novel nanosilver-based antimicrobial agents exhibiting safety, stability, and superior antimicrobial efficacy was recognized as imperative. Carboxylated chitosan and pectin were utilized as the primary raw materials. Silver nanoparticles based on carboxylated chitosan-pectin composites (CP-AgNPs) and pectin (P-AgNPs) were synthesized via a microwave-assisted method. The synthesis conditions for silver nanoparticles (AgNPs) were optimized through single-factor experiments. Structural characterization of AgNPs was conducted via Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Zeta potential analysis, X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The antioxidant activity was further evaluated. The optimal synthesis conditions were identified through this comprehensive approach as requiring a microwave power of 480 W, a temperature of 60 °C, a duration of 4 min, and a silver nitrate concentration of 0.7 mol/L. These parameters were subsequently applied in the synthesis of CP-AgNPs and P-AgNPs, both of which were characterized by the surface plasmon resonance absorption peak of AgNPs at 410 nm. The spherical morphology of both CP-AgNPs and P-AgNPs was characterized by SEM analysis, with respective diameters measuring 33.18 nm and 19.44 nm. Face-centered cubic crystalline structures were identified in both types through XRD analysis, where CP-AgNPs exhibited enhanced colloidal stability compared to P-AgNPs as evidenced by Zeta potential measurements. The synthesis conditions for CP-AgNPs were actively investigated, and their antioxidant activity was rigorously evaluated. The highest radical scavenging rates were demonstrated as 85.30% for DPPH, 63.35% for hydroxyl radicals, and 54.10% for ABTS+. Based on these findings, a stable and eco-friendly nanosilver-based antioxidant agent was successfully synthesized via microwave-assisted methods, with its efficacy validated through comparative experiments against ascorbic acid standards. This novel material was further confirmed to hold significant potential for applications in food preservation and pharmaceutical formulations, particularly in mitigating oxidative stress-related cellular damage.

     

/

返回文章
返回