Abstract: Selenopolysaccharides derived from auricularia fruiting bodies were isolated and structurally characterized to advance their research and application. Through optimization, the highest polysaccharide yield (11.62%) was achieved under conditions of 1:60 solid-liquid ratio, 50 ℃, and 3 h extraction time. The conversion efficiency of selenated Auricularia to inorganic selenium was measured at 88.90%. The spectral analysis of selenium polysaccharides revealed characteristic absorption peaks corresponding to Se=O, Se-O and other functional groups. The surface morphology of selenated auricularia exhibited a well-organized network structure with minimal alteration in crystal morphology. Additionally, the antioxidant capacity of selenopolysaccharides was significantly (P<0.05) enhanced compared to non-selenized polysaccharides. At a concentration of 5 mg/mL, selenopolysaccharides demonstrated an 18.80% increase in inhibitory activity against α-glucosidase and an 8.30% increase against α-amylase, as well as a cholesterol adsorption rate of 61.40%. These findings provide valuable theoretical and technical insights for the development of auricularia selenopolysaccharide products.