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中国精品科技期刊2020
胡晓霞,刘晓丹,许萍,等. 基于Co3O4-NC的过氧化氢酶活性比色检测茶叶中的没食子酸[J]. 食品工业科技,2025,46(23):302−309. doi: 10.13386/j.issn1002-0306.2024070256.
引用本文: 胡晓霞,刘晓丹,许萍,等. 基于Co3O4-NC的过氧化氢酶活性比色检测茶叶中的没食子酸[J]. 食品工业科技,2025,46(23):302−309. doi: 10.13386/j.issn1002-0306.2024070256.
HU Xiaoxia, LIU Xiaodan, XU Ping, et al. Colorimetric Detection of Gallic Acid in Tea Based on the Catalase-like Activity of Co3O4-NC Nanozyme[J]. Science and Technology of Food Industry, 2025, 46(23): 302−309. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024070256.
Citation: HU Xiaoxia, LIU Xiaodan, XU Ping, et al. Colorimetric Detection of Gallic Acid in Tea Based on the Catalase-like Activity of Co3O4-NC Nanozyme[J]. Science and Technology of Food Industry, 2025, 46(23): 302−309. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024070256.

基于Co3O4-NC的过氧化氢酶活性比色检测茶叶中的没食子酸

Colorimetric Detection of Gallic Acid in Tea Based on the Catalase-like Activity of Co3O4-NC Nanozyme

  • 摘要: 目的:本文旨在开发一种基于四氧化三钴-氮碳共掺杂复合纳米材料(Co3O4-NC)的过氧化氢酶活性比色检测茶叶中没食子酸(Gallic acid,GA)的定量分析方法。方法:通过在金属有机框架材料二甲基咪唑钴(Zeolitic imidazolate frameworks-67,ZIF-67)表面自聚形成聚多巴胺,高温热解将N元素、C元素引入到Co3O4纳米结构中得到了具有高过氧化氢酶活性的纳米酶Co3O4-NC。在H2O2存在下,Co3O4-NC能够催化氧化无色的3,3’,5,5’-四甲基联苯胺(Tetramethylbenzidine,TMB)生成蓝色的氧化态产物oxTMB,没食子酸的加入可有效地抑制这一反应,从而构建了一种基于Co3O4-NC过氧化氢酶活性的比色传感平台用于测定GA。结果:该方法用于检测没食子酸的线性工作范围为0.25~20.00 μmol/L,回归方程为ΔA=0.0310C+0.0384(R2=0.995),检出限(Limit of detection,LOD,3σ/S)为0.077 μmol/L,定量限(Limit of quantitation,LOQ,10σ/S)为0.26 μmol/L。结论:将该方法用于检测市售绿茶、红茶和乌龙茶中没食子酸当量,分别为2.33%、1.82%及0.46%,加标回收率在90.97%~105.22%之间,RSD<5%,表明该比色检测方法具有良好的应用前景。

     

    Abstract: Objective: The present study was aimed to develop a rapid colorimetric method for the detection of gallic acid in tea based on the catalase-like activity of Co3O4-NC nanozyme. Methods: The Co3O4-NC was prepared by calcination of the composite material of zeolitic imidazolate frameworks-67 (ZIF-67) and polydopamine. The letramethylbenzidine (TMB) could be oxidized to blue oxTMB in the presence of H2O2 by Co3O4-NC nanozyme, while the addition of gallic acid would restrain the catalase-like activity and the color of the solution will be lighter. Based on the mechanism, a colorimetric sensing platform for the detection of gallic acid was established. Results: The linear curve between ΔA and GA concentration was observed within the range of 0.25~20.00 μmol/L, the linear equation was ΔA=0.0310C+0.0384 (R2=0.995), the limit of detection (LOD, 3σ/S) was 0.077 μmol/L and the limit of quantitation (LOQ, 10σ/S) was 0.26 μmol/L. Conclusion: The method could be applied to detect gallic acid equivalent (GAE) in tea, and the GAE in green tea, black tea and oolong tea were tested as 2.33%, 1.82%, and 0.46%, respectively. The recovery rates ranged from 90.97% to 105.22% with RSD<5%, indicating that the colorimetric method had a good application prospect.

     

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