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中国精品科技期刊2020
付亿芬,刘雨晗,敬乐,等. β-胡萝卜素-碳酸钙色淀的制备工艺优化及表征分析[J]. 食品工业科技,xxxx,x(x):1−10. doi: 10.13386/j.issn1002-0306.2024070323.
引用本文: 付亿芬,刘雨晗,敬乐,等. β-胡萝卜素-碳酸钙色淀的制备工艺优化及表征分析[J]. 食品工业科技,xxxx,x(x):1−10. doi: 10.13386/j.issn1002-0306.2024070323.
FU Yifen, LIU Yuhan, JING Le, et al. Process Optimization and Characterization of β-Carotene-Calcium Carbonate Lake[J]. Science and Technology of Food Industry, xxxx, x(x): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024070323.
Citation: FU Yifen, LIU Yuhan, JING Le, et al. Process Optimization and Characterization of β-Carotene-Calcium Carbonate Lake[J]. Science and Technology of Food Industry, xxxx, x(x): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024070323.

β-胡萝卜素-碳酸钙色淀的制备工艺优化及表征分析

Process Optimization and Characterization of β-Carotene-Calcium Carbonate Lake

  • 摘要: 脂溶性色素通常来源于天然提取物,具有较高的安全性和多样的生理功能,但由于其化学性质活泼,稳定性较差。为了提高脂溶性色素的稳定性,本文以β-胡萝卜素为代表,首次探索了色淀技术在脂溶性色素中的应用。实验制备了β-胡萝卜素-碳酸钙色淀,通过单因素实验和响应曲面法对其制备工艺进行了优化,并采用激光粒度分析、SEM、XRD和拉曼光谱等技术进行表征分析。结果显示,β-胡萝卜素-碳酸钙色淀的最佳制备条件为:pH9.9,剪切速度15400 r/min,分散剪切时间60 s,共沉淀剪切时间120 s,温度45 ℃。在此条件下制备的色淀吸附率为23.525 mg/g。粒径分析结果表明,剪切作用显著降低了β-胡萝卜素悬浮微粒的粒径,有利于色淀的形成。色淀中的碳酸钙呈现方解石的形貌特征,剪切作用导致部分颗粒破碎。XRD分析确定了色淀中碳酸钙为方解石。拉曼光谱进一步确认了色淀中碳酸钙的晶型,图谱对比结果表明,色淀中β-胡萝卜素与碳酸钙之间是通过非化学键结合的。总之,在优化条件下,碳酸钙以方解石形式有效负载β-胡萝卜素,剪切操作显著降低了色素微粒的粒径,这些微粒与碳酸钙通过非化学作用力结合。本研究为脂溶性色素色淀的开发提供了参考,为提升脂溶性色素的稳定性提供了新的技术路径。

     

    Abstract: Liposoluble pigments, predominantly sourced from natural extracts, are celebrated for their exceptional safety profile and a wide array of physiological benefits. Nevertheless, their inherent chemical reactivity compromises their stability, posing significant challenges for their application. Addressing this issue, the current study investigated the efficacy of lake technology, employing β-carotene as a prototypical pigment, to bolster the stability of liposoluble pigments. β-Carotene-calcium carbonate lakes were prepared, with the preparation process optimized through single-factor experiments and response surface methodology. Comprehensive characterization techniques, including laser particle size analysis, SEM, XRD, and Raman spectroscopy, were employed to elucidate the physicochemical properties of the synthesized lake. The findings delineated that the ideal conditions for the formation of β-carotene-calcium carbonate lakes encompassed a pH of 9.9, a shear speed of 15400 r/min, a dispersion shear duration of 60 s, a co-precipitation shear time of 120 s, and a temperature of 45 ℃. Under these optimized parameters, the lake demonstrated an impressive adsorption capacity of 23.525 mg/g. Notably, the shear process markedly diminished the particle size of the β-carotene suspensions, thereby facilitating the efficient formation of the lake. The calcium carbonate within the lake predominantly exhibited the calcite crystal morphology, with some particles showing signs of fracturing due to the applied shear forces. XRD analysis confirmed calcite as the crystalline form of the calcium carbonate present in the lake. Furthermore, Raman spectroscopy corroborated this finding and revealed the absence of chemical bonds between β-carotene and calcium carbonate, suggesting the predominance of non-covalent interactions. In summary, the study demonstrated that under carefully optimized conditions, calcium carbonate, presented in the calcite form, served as an effective carrier for β-carotene, with shear processes playing a pivotal role in reducing the particle size of the pigments. These pigments were anchored to calcium carbonate via non-covalent forces, presenting a promising avenue for the development of liposoluble pigment lakes. This research not only provides a valuable reference for the fabrication of lakes for liposoluble pigments, but also introduces a novel methodology for the enhancement of the stability of these pigments.

     

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