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中国精品科技期刊2020
柯美伶,韩帅,朱联旭,等. 基于非靶向代谢组学分析镇巴腊肉熏制过程中代谢物的动态变化规律[J]. 食品工业科技,2025,46(12):1−9. doi: 10.13386/j.issn1002-0306.2024070417.
引用本文: 柯美伶,韩帅,朱联旭,等. 基于非靶向代谢组学分析镇巴腊肉熏制过程中代谢物的动态变化规律[J]. 食品工业科技,2025,46(12):1−9. doi: 10.13386/j.issn1002-0306.2024070417.
KE Meiling, HAN Shuai, ZHU Lianxu, et al. Analysis of the Dynamic Metabolic Changes during the Smoking Process of the Zhenba Bacon Based on Non-targeted Metabolomics[J]. Science and Technology of Food Industry, 2025, 46(12): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024070417.
Citation: KE Meiling, HAN Shuai, ZHU Lianxu, et al. Analysis of the Dynamic Metabolic Changes during the Smoking Process of the Zhenba Bacon Based on Non-targeted Metabolomics[J]. Science and Technology of Food Industry, 2025, 46(12): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024070417.

基于非靶向代谢组学分析镇巴腊肉熏制过程中代谢物的动态变化规律

Analysis of the Dynamic Metabolic Changes during the Smoking Process of the Zhenba Bacon Based on Non-targeted Metabolomics

  • 摘要: 镇巴腊肉是陕西省的一种传统腌腊肉制品。为探究镇巴腊肉在熏制过程中的代谢物组成和动态变化规律,本研究基于非靶向代谢组学,对熏制前期(8、16 d)、中期(24 d)和后期(32、40 d)的腊肉进行分析,初步探讨差异代谢物与腊肉风味形成的关系。结果表明,鉴定出94种共表达的差异代谢物。KEGG富集分析发现氨基酸、脂质代谢是主要的代谢途径。进一步对腊肉熏制各阶段代谢物的时间表达趋势分析发现,随着熏制时间的延长,镇巴腊肉中的有机杂环化合物(例如乌药碱)含量逐渐减少,而对腊肉风味有显著贡献的氨基酸和脂质代谢物(如精氨酸、赖氨酸和胆固醇)含量则逐渐增加。综上所述,熏制时间的延长有助于提升镇巴腊肉的风味物质含量,且安全性和质量也逐渐提高。本研究结果为优化镇巴腊肉的传统加工工艺提供了科学依据,同时为该产品实现现代化的安全生产提供了技术参考。

     

    Abstract: Zhenba bacon is a traditional preserved meat product in Shaanxi Province. The metabolite composition and dynamic changes of Zhenba bacon during the smoking process were systematically explored through non-targeted metabolomics analysis. Bacon samples were categorized and analyzed across three distinct processing stages: early (8 and 16 days), middle (24 days), and late (32 and 40 days) stages. The relationship between various metabolites and the formation of bacon flavor was preliminarily investigated, with particular attention given to the temporal evolution of metabolic profiles. Results revealed that 94 different co-expressed metabolites were identified. KEGG enrichment analysis revealed that amino acid metabolism and lipid metabolism were identified as the primary metabolic pathways. Further analysis of the temporal expression trends of metabolites throughout the smoking process revealed a gradual decrease in the content of organic heterocyclic compounds (e.g., Coclaurine) with prolonged smoking duration. Conversely, the concentrations of amino acids and lipid metabolites (e.g., arginine, lysine, and cholesterol), which played a significant role in flavor development, exhibited a progressive increase during the smoking process. In summary, prolonged smoking duration enhanced the flavor substance content of Zhenba bacon while progressively improving its safety and quality. A scientific foundation was established for optimizing the traditional processing technology of Zhenba bacon, while critical insights were provided for the adoption of modern safety protocols in production practices.

     

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