Abstract: In order to explore the effect of ultraviolet C (UV-C) treatment combined with near-freezing temperature (NFT) storage on preservation and physiological indices of prunes, Xinjiang 'France' prunes was used as material. The samples underwent treatment consisting of a 2.0 kJ/m² dose of UV-C irradiation, storage at near-freezing temperature −1.5±0.5 °C storage, and a combination of both treatments (UV-C+NFT). The storage conditions were as follows: Relative humidity 90%~95%, duration of 80 days. Quality indexes and antioxidant oxidase activity indexes were measured regularly. The results showed that UV-C, NFT and UV-C+NFT significantly reduced the rate of weight loss and decay, delayed the decrease of fruit firmness, titrable acid (TA) content, and total soluble solid (TSS) during the storage period compared with the control group. Furthermore, the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) were enhanced while suppressing the accumulation of malondialdehyde (MDA) content and the increase in cell membrane permeability in prune fruits of each treatment group. In addition, all treatment groups promoted the activities of phenylalamine ammonia (PAL), improved the contents of total polyphenols and flavonoids, in which the effect of UV-C+NFT treatment was better than NFT group. At the end of storage, NFT treatment inhibited the activity of polyphenol oxidase (PPO), and UV-C treatment increased the activity of PPO and delayed the peak time of PPO activity in prune fruits. Therefore, ultraviolet C treatment combined with near-freezing temperature storage effectively maintained the quality of prunes, enhanced the antioxidant oxidase activity, which is an effective preservation method to improve the post-harvest storage quality of prunes.