Abstract: Objective: The intervention effect of Canna edulis high fiber meal replacement powder(CMRP) on the obesity in high-fat diet-induced hyperlipidemic mice was explored in this paper. Methods: Forty mice were randomly divided into control group, high-fat diet group, positive group, low-dose CMRP group, and high-dose CMRP group. The intervention was carried out for 8 weeks. Body weight, organ weight, and weekly food intake, lipid levels, liver injury levels and antioxidant levels of mice were measured. Results: The characteristics of high dietary fiber and protein contents were possessed by CMRP, with dietary fiber content quantified as (24.03±0.07) g/100 g CMRP and protein content as (12.80±0.02) g/100 g CMRP, respectively. After intervention trial for 8 weeks, compared with high-fat diet group, it was found that high-dose CMRP group could significantly reduce the body weight and abdominal fat weight of mice (P<0.05). It was indicated that a synergistic effect on weight reduction was exhibited by appropriate food intake and nutritional components. Compared with high-fat diet group, CMRP intervention reduced significantly the serum cholesterol and aspartate aminotransferase contents (P<0.05) of mice. The low-density lipoprotein content in low-dose and high-dose CMRP groups was significantly reduced by 44.60% and 39.92% (P<0.05), as well as their high-density lipoprotein content was significantly increased by 21.30% and 61.19% (P<0.05), compared with high-fat diet group. Additionally, compared with high-fat diet group, the fecal moisture content in CMRP groups was increased, and their pH was decreased, leading to improve intestinal microecology system of high-fat diet-induced hyperlipidemic mice. Liver antioxidant enzymatic activity and lipid hydroperoxide content were also positively regulated by the CMRP diet, resulting in a significant decrease in oxidative damage to liver tissue induced by a high-fat diet (P<0.05). Conclusion: A significant obesity-intervention effect on high-fat diet was demonstrated by the formula powder in this study, through positive regulation of lipid levels and intestinal microecology, combined with a reduction in oxidative damage. Consequently, CMRP could be utilized as a functional food for auxiliary reduction of high-fat diet-induced damage. Practical guidance for the development of Canna edulis replacement powder (CMRP) was provided by this research.