Abstract: In this paper, four protein-EGCG non-covalent complexes were prepared by EGCG and protein (soybean protein isolate, casein, egg albumin and β lactoglobulin) to investigate effects of nutrient supplementation on the anti-aging activity of protein-epigallocatechin gallate (EGCG) non-covalent complex. With the inhibiting rates of tyrosinase, collagens, elastase and hyaluronidase as the anti-aging indicators in vitro, the anti-aging properties of four protein-EGCG non-covalent complexes were compared to select the proper carrier for exploring effects of nutrient addition (single or compound addition of soybean oil, sucrose and calcium) on the anti-aging activities of protein-EGCG non-covalent complex. The results showed that soybean protein isolate-EGCG non-covalent complex had higher inhibitory rates of tyrosinase, elastase and collagenase than others, which would be significantly increased after nutrient supplementation (P<0.05). Among them, the increase of elastase inhibitory rate was highest, followed by collagenase inhibitory rate, and tyrosinase inhibitory rate was the lowest. Effects of nutrients on anti-aging activities in vitro of protein-EGCG non-covalent complex varied with the type and composition of nutrients. In general, the anti-aging activity of EGCG could be improved after its non-covalent binding to protein, the addition of sucrose, calcium and soybean oil into protein-EGCG non-covalent complex was prone to have superior anti-aging activity in vitro.