Abstract: A high performance liquid chromatography-diode array detector (HPLC-DAD) method was developed for the simultaneous determination of 28 bisphenols (BPs) in dairy products, based on organic solvent protein precipitation. This method provides technical support for assessing BPs contamination risks and enhancing food safety regulation. The dairy products were extracted with acetonitrile containing 0.1% formic acid, followed by freezing centrifugation for lipid removal, and filtered through 0.22 μm polytetrafluoroethylene (PTFE) membrane. The target analytes were separated on an InfinityLab Poroshell 120 EC-C₁₈ column by a gradient separation using a mixture of 0.1% phosphoric acid and acetonitrile as the mobile phase, and then determined by diode array detector (DAD). The detection wavelength was 230 nm, the column temperature was 40 ℃, the flow rate was 1.0 mL/min, and the injection volume was 10 μL. The experiment investigated the effects of extraction solvent, NaCl addition amount, and extraction cycles on BPs quantification. The results showed that 28 kinds of BPs were effectively separated within 17 min. All 28 BPs demonstrated excellent linearity in their respective concentration ranges, with decision coefficients (R²) exceeding 0.9990 for all calibration curves. The limits of detection (LODs) were 0.01~0.05 mg/kg, the limits of quantification (LOQs) were 0.02~0.10 mg/kg, the average recoveries were 84.2%~103.1%, and the relative standard deviations (RSDs) were 0.6%~6.3% (n=6). The method is convenient, rapid, efficient, accurate and reliable, which is suitable for quantitative detection of 28 BPs in dairy products.