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中国精品科技期刊2020
贾庆超,谷焕焕,张辉艳,等. 保加利亚乳杆菌和嗜热链球菌协同发酵制备液态黑蒜技术研究[J]. 食品工业科技,2026,47(3):1−12. doi: 10.13386/j.issn1002-0306.2025010286.
引用本文: 贾庆超,谷焕焕,张辉艳,等. 保加利亚乳杆菌和嗜热链球菌协同发酵制备液态黑蒜技术研究[J]. 食品工业科技,2026,47(3):1−12. doi: 10.13386/j.issn1002-0306.2025010286.
JIA Qingchao, GU Huanhuan, ZHANG Huiyan, et al. Study on the Preparation Technology of Liquid Black Garlic by Synergistic Fermentation of Lactobacillus Bulgaricus and Streptococcus Thermophilus[J]. Science and Technology of Food Industry, 2026, 47(3): 1−12. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025010286.
Citation: JIA Qingchao, GU Huanhuan, ZHANG Huiyan, et al. Study on the Preparation Technology of Liquid Black Garlic by Synergistic Fermentation of Lactobacillus Bulgaricus and Streptococcus Thermophilus[J]. Science and Technology of Food Industry, 2026, 47(3): 1−12. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025010286.

保加利亚乳杆菌和嗜热链球菌协同发酵制备液态黑蒜技术研究

Study on the Preparation Technology of Liquid Black Garlic by Synergistic Fermentation of Lactobacillus Bulgaricus and Streptococcus Thermophilus

  • 摘要: 目的:本文以新鲜大蒜为原料,研究乳酸菌协同发酵制备液态黑蒜技术及对其香气成分进行分析。方法:将新鲜大蒜经180 ℃高温灭酶,并破碎、加水,加入不同比例复合乳酸菌发酵一定时间,于80 ℃高温热反应制备液态黑蒜,以未加菌种发酵的液态黑蒜(KB1、KB2)为参照,每隔2 d测定其褐变程度、还原糖、总酚、总酸、氨基酸态氮含量及DPPH·、·OH清除率,研究在不同比例复合乳酸菌发酵作用下,制备液态黑蒜的最佳条件,并对此条件下的液态黑蒜进行香气成分分析。结果:液态黑蒜最佳制备条件为:灭酶时间9 min,乳酸菌质量比例嗜热链球菌(S):保加利亚乳杆菌(B)为3:1,热反应13 d达到黑蒜成熟标准,以相同条件下KB1为参照,此时还原糖含量为其1.20倍,总酚含量为其1.82倍,总酸含量为其1.36倍,氨基酸态氮为其1.21倍,DPPH·清除率和·OH清除率分别为其1.37、1.34倍。相对单一乳酸菌S和B,发酵时间分别缩短2 d和5 d。香气成分分析表明,相对KB1,菌种协同发酵的液态黑蒜硫醚类物质含量降低54.17%,醛类、呋喃类挥发性香气成分分别增加47.98%和6.18%,液态黑蒜基本没有蒜臭味,香气可接受度更好,感官评分为91.38±0.98分。结论:复合乳酸菌发酵制备液态黑蒜具有协同作用,可缩短黑蒜成熟时间,在黑蒜开发研究中具有良好应用前景。

     

    Abstract: Objective: The preparation of liquid black garlic by lactic acid bacteria cooperative fermentation and the analysis of aroma components were studied. Methods: Fresh garlic was subjected to high-temperature enzyme inactivation at 180 ℃, then crushed and mixed with water. Different proportions of composite lactic acid bacteria were added for fermentation for a certain period of time. Liquid black garlic was prepared by high-temperature reaction at 80 ℃. The degree of browning, reducing sugar, total phenols, total acids, amino acid nitrogen content and DPPH·, ·OH clearance rate were measured every two days using liquid black garlic (KB1、KB2) without bacterial fermentation as a reference, and the optimum conditions for preparing liquid black garlic were studied under the fermentation of complex lactic acid bacteria in different proportions, and the aroma components of liquid black garlic under these conditions were analyzed. Results: The best preparation conditions for liquid black garlic were the fermentation time was 9 min, the mass ratio of lactic acid bacteria S (Streptococcus thermophilus):B (Lactobacillus bulgaricus) was 3:1, and the maturation standard of black garlic was reached at 13 d. Under the same conditions, KB1 was used as the standard. At this time, the reducing sugar content was 1.20 times, the total phenol content was 1.82 times, the total acid content was 1.36 times, and the amino acid nitrogen was 1.21 times. DPPH· clearance and ·OH clearance were 1.37 times and 1.34 times, respectively. Compared with single lactic acid bacteria S and B, the thermal reaction was shortened by 2 d and 5 d, respectively. The analysis of aroma components showed that, compared with KB1, the content of thioether in liquid black garlic co-fermented by strains decreased by 54.17%, while the volatile aroma components of aldehydes and furan compounds increased by 47.98% and 6.18% respectively. The liquid black garlic basically had no garlic odor, and the aroma acceptability was better, with a sensory score of 91.38±0.98 points. Conclusion: The fermentation of liquid black garlic by complex lactic acid bacteria has a synergistic effect and can shorten the thermal reaction of black garlic, which has a good application prospect in the development and research of black garlic.

     

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