Abstract: To investigate the effect of low-temperature plasma-activated water (PAW) on the storage quality of postharvest Dendrocalamus brandisii shoots (D. brandisii), using low-temperature plasma at 160 V and 160 Hz, preparation of PAWs (PAW₁, PAW₂, PAW₃) at 1, 2, and 3 min, the changes of PAW on the total number of colonies, enzyme activities and related physicochemical indexes of D. brandisii shoots after harvest were analyzed. The results show that compared to the CK group (distilled water treatment), PAW could inhibit the respiratory intensity of fresh-cut D. brandisii shoots, to reduce the loss of total phenols and flavonoids, delayed decline in soluble protein, soluble sugar, vitamin C content. PAW₁, PAW₂, and PAW₃-treated D. brandisii shoots at the 18th day of storage, the activity of phenylalanine ammonia-lyase decreased by 3.00, 5.25 and 3.90 U/g (P<0.05). On the 12th day of storage, peroxidase activity decreased by 5.91, 10.16 and 7.94 U/g (P<0.05). On the 30th day of storage, the cumulative synthesis of cellulose and lignin decreased by 6.66, 24.19 and 10.59 mg/g (P<0.05) and 1.27, 2.49 and 1.20 mg/g, respectively (P<0.05). This study provides some theoretical basis for the application of PAW technology in the field of preservation and processing of D. brandisii shoots.