Abstract: Objective: To investigate the effects of zinc-enriched probiotic preparations on gastrointestinal hormones and gastrointestinal mucosa in rats with functional dyspepsia (FD). Methods: The FD model rats were established via intragastric administration of iodoacetamide-sucrose solution, exhaustive swimming stress, and intermittent fasting. Mosapride citrate served as the positive control. The efficacy of zinc-enriched probiotic preparations in alleviating FD was systematically evaluated through behavioral observation, biochemical analysis, pathological examination, and detection of related genes in gastrointestinal tissues. Results: Compared with the model group, zinc-enriched probiotic preparations significantly increased body weight and food intake in FD rats (P<0.05); decreased serum levels of 5-hydroxytryptamine (5-HT), gastrin (Gas), and cholecystokinin (CCK) (P<0.01), while increasing serum levels of substance P (SP) and amylase (AMS) (P<0.001). They also enhanced pepsin and trypsin activity (P<0.05). Histopathological examination revealed that the villus length and villus length-to-crypt depth ratio in the duodenum and jejunum were significantly increased (P<0.05). The expression of tight junction proteins (Cldn1, Tjp1) in the duodenum and the expression of tyrosine kinase receptor (c-Kit) and stem cell factor (SCF) in the gastric tissues were significantly upregulated (P<0.01). Conclusion: Zinc-enriched probiotic preparations can effectively alleviate FD by increasing digestive enzyme activity, regulating gastrointestinal hormone levels, enhancing gastrointestinal motility, and repairing the mucosal barrier (through upregulation of tight junction proteins and activation of the stem cell factor pathway). They have potential clinical application value and are worthy of further in-depth research.