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中国精品科技期刊2020
刘瑜,葛红娟,张鑫鑫,等. 30种海棠果的综合品质分析及其黄嘌呤氧化酶抑制活性的探究J. 食品工业科技,2026,47(6):1−12. doi: 10.13386/j.issn1002-0306.2025030055.
引用本文: 刘瑜,葛红娟,张鑫鑫,等. 30种海棠果的综合品质分析及其黄嘌呤氧化酶抑制活性的探究J. 食品工业科技,2026,47(6):1−12. doi: 10.13386/j.issn1002-0306.2025030055.
LIU Yu, GE Hongjuan, ZHANG Xinxin, et al. Comprehensive Quality Analysis and Exploration of Xanthine Oxidase Inhibitory Activity of 30 Crabapple VarietiesJ. Science and Technology of Food Industry, 2026, 47(6): 1−12. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025030055.
Citation: LIU Yu, GE Hongjuan, ZHANG Xinxin, et al. Comprehensive Quality Analysis and Exploration of Xanthine Oxidase Inhibitory Activity of 30 Crabapple VarietiesJ. Science and Technology of Food Industry, 2026, 47(6): 1−12. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025030055.

30种海棠果的综合品质分析及其黄嘌呤氧化酶抑制活性的探究

Comprehensive Quality Analysis and Exploration of Xanthine Oxidase Inhibitory Activity of 30 Crabapple Varieties

  • 摘要: 为更好地了解不同品种海棠果的品质特性,有效利用海棠果资源,本研究测定了30个品种海棠果的单果重、水分含量、可溶性固形物等理化指标及黄嘌呤氧化酶半抑制浓度等生物活性指标,通过测定DPPH自由基清除能力、ABTS+自由基清除能力和铁离子还原能力比较了体外抗氧化活性,并对其进行了相关性分析、主成分分析和聚类分析。结果表明,所测品种海棠果中总酚含量(2.749~80.877 mg GAE/g DW)和总黄酮含量(3.522~167.312 mg RE/g DW)较高,和果实横径、纵径、单果重呈显著负相关(P<0.05),具有较好的抗氧化活性和黄嘌呤氧化酶抑制活性。经主成分分析,5种主成分累计贡献率为82.570%,海棠果实综合评价模型筛选出‘2010-6’为品质最佳的品种,其总酚、总黄酮含量在所测品种中最高,且具有较好的抗氧化活性(0.827、0.654和0.518 mmol TE/g DW)和较强的黄嘌呤氧化酶抑制活性(IC50=2.724 mg/mL),具有较好的开发利用的前景。海棠果的糖酸比(0.47~5.48)较小,更适宜用来加工制成果汁、果酒等产品,或作为提取活性物质的来源。本研究从营养品质、功能特性等方面对海棠果进行了全面的分析,为不同品种海棠果的开发利用提供理论依据。

     

    Abstract: To elucidate the quality characteristics of crabapple cultivars and facilitate resource utilization, we systematically evaluated 30 crabapple varieties using multidimensional analyses. Physicochemical parameters (fruit weight, moisture content, and soluble solids content) and bioactive profiles (xanthine oxidase inhibitory activity, expressed as IC50) were quantified. Antioxidant potential was assessed using three established methods: DPPH and ABTS+ radical scavenging capacity, and ferric ion-reducing antioxidant power. Multivariate statistical approaches, including correlation analysis, principal component analysis (PCA), and hierarchical cluster analysis, were used for data interpretation. Notably, the cultivars contained substantial levels of total phenolics (2.749~80.877 mg GAE/g DW) and flavonoids (3.522~167.312 mg RE/g DW), demonstrating strong negative correlations (P<0.05) with fruit morphometric parameters (diameter, length, and weight). These phytochemical profiles accounted for the remarkable antioxidant capacity and inhibition of xanthine oxidase. The PCA revealed that the first five principal components explained 82.570% of the total variance. Through the established quality evaluation model, cultivar '2010-6' emerged as the most promising candidate, characterized by the highest phenolic and flavonoid contents, superior antioxidant activities (0.827, 0.654, and 0.518 mmol TE/g DW), and potent xanthine oxidase inhibition (IC50 2.724 mg/mL). Despite the suboptimal sugar–acid ratio (0.47~5.48), these findings position crabapples as valuable raw materials for functional food processing (such as juice and wine production) and natural bioactive compound extraction. This study establishes a scientific framework for the comprehensive quality assessment of crabapple resources, providing critical insights for varietal selection in nutraceutical development and value-added processing.

     

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