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中国精品科技期刊2020
朱向巍,李媛媛,航盖,等. 戈壁红驼肉宰后成熟过程中品质及蛋白质变化研究J. 食品工业科技,2026,47(7):1−10. doi: 10.13386/j.issn1002-0306.2025030196.
引用本文: 朱向巍,李媛媛,航盖,等. 戈壁红驼肉宰后成熟过程中品质及蛋白质变化研究J. 食品工业科技,2026,47(7):1−10. doi: 10.13386/j.issn1002-0306.2025030196.
ZHU Xiangwei, LI Yuanyuan, HANG Gai, et al. Investigation of Protein Changes and Quality Attributes in Gobi Red Camel Meat during Postmortem AgingJ. Science and Technology of Food Industry, 2026, 47(7): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025030196.
Citation: ZHU Xiangwei, LI Yuanyuan, HANG Gai, et al. Investigation of Protein Changes and Quality Attributes in Gobi Red Camel Meat during Postmortem AgingJ. Science and Technology of Food Industry, 2026, 47(7): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025030196.

戈壁红驼肉宰后成熟过程中品质及蛋白质变化研究

Investigation of Protein Changes and Quality Attributes in Gobi Red Camel Meat during Postmortem Aging

  • 摘要: 为探究戈壁红驼肉宰后成熟过程中品质和蛋白质的变化规律,测定宰后成熟0、1、3、5、7 d品质指标(pH、肉色、剪切力、蒸煮损失、质构、脂肪酸含量)及蛋白质特性变化(十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、总羰基含量、巯基含量、肌原纤维小片化指数(MFI)及肌原纤维蛋白二级结构)。采用Pearson相关分析法解析成熟时间与各指标间的关联性。结果表明,随着成熟时间延长,驼肉pH先下降后上升,3 d时最低;剪切力、硬度、咀嚼性先升高后降低,3 d时最高;蒸煮损失、L*值、a*值先上升后下降;b*值逐渐上升,7 d时达到14.36±1.29;饱和脂肪酸(SFA)先升高后降低,5 d时最高,其相对含量为47.08%±0.12%;单不饱和脂肪酸(MUFA)先下降后上升;多不饱和脂肪酸(PUFA)先升高后下降,5 d时最低,其相对含量降至16.80%±0.13%。蛋白质特性方面,总羰基含量和MFI持续上升,巯基含量下降,SDS-PAGE显示3 d时出现新蛋白条带,且成熟时间越长条带越明显;二级结构中α-螺旋和β-折叠向无规则卷曲和β-转角转化。相关性分析结果显示,成熟时间与总羰基含量、MFI等呈极显著正相关(P<0.001),与β-折叠、剪切力等呈极显著负相关(P<0.001)。综上,驼肉适宜成熟时间为3~5 d,此时驼肉品质特性最佳。

     

    Abstract: This study investigated the changes in quality attributes and protein characteristics of Gobi red camel meat during post-slaughter maturation (0, 1, 3, 5, and 7 d). Quality parameters including pH, meat color, shear force, cooking loss, texture profile, and fatty acid composition were analyzed. Protein characteristics were evaluated through SDS-PAGE, total carbonyl content, sulfhydryl content, myofibrillar fragmentation index (MFI), and myofibrillar protein secondary structure. Pearson correlation analysis was performed to examine relationships between maturation time and measured indices. The results showed that the pH exhibited a U-shaped trend, reaching its lowest point at 3 d. Shear force, hardness, and chewiness followed a similar pattern, peaking at 3 d. Cooking loss, L* (lightness), and a* (redness) initially increased before decreasing, while b* (yellowness) progressively increased to 14.36±1.29 at 7 d. Fatty acid composition showed dynamic changes. Saturated fatty acids (SFA) peaked at 5 d (47.08%±0.12%). Monounsaturated fatty acids (MUFA) demonstrated a trough pattern. Polyunsaturated fatty acids (PUFA) reached their lowest level at 5 d (16.80%±0.13%). Protein analysis revealed that progressive increase in total carbonyl content and MFI. Decline in sulfhydryl group content. Emergence of new protein bands in SDS-PAGE from 3 d onward. Secondary structure transitions from α-helix and β-sheet to irregular coils and β-turns. Correlation analysis revealed that maturation time exhibited a strong positive correlation with both total carbonyl content and MFI (P<0.001), while showing a highly significant negative correlation with β-sheet content and shear force (P<0.001). Conclusion,the optimal maturation period for camel meat was determined to be 3~5 d, when the meat exhibited its most favorable quality characteristics.

     

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