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中国精品科技期刊2020
陈钰洁,李蒙,刘群,等. 基于磁感应电场-酶解耦合技术的鲍鱼内脏牛磺酸提取、纯化及表征J. 食品工业科技,2026,47(9):113−122. doi: 10.13386/j.issn1002-0306.2025040065.
引用本文: 陈钰洁,李蒙,刘群,等. 基于磁感应电场-酶解耦合技术的鲍鱼内脏牛磺酸提取、纯化及表征J. 食品工业科技,2026,47(9):113−122. doi: 10.13386/j.issn1002-0306.2025040065.
CHEN Yujie, LI Meng, LIU Qun, et al. Magnetically Induced Electric Field-Enzymatic Hydrolysis Coupled Extraction, Purification and Characterization of Taurine from Abalone VisceraJ. Science and Technology of Food Industry, 2026, 47(9): 113−122. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025040065.
Citation: CHEN Yujie, LI Meng, LIU Qun, et al. Magnetically Induced Electric Field-Enzymatic Hydrolysis Coupled Extraction, Purification and Characterization of Taurine from Abalone VisceraJ. Science and Technology of Food Industry, 2026, 47(9): 113−122. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025040065.

基于磁感应电场-酶解耦合技术的鲍鱼内脏牛磺酸提取、纯化及表征

Magnetically Induced Electric Field-Enzymatic Hydrolysis Coupled Extraction, Purification and Characterization of Taurine from Abalone Viscera

  • 摘要: 以鲍鱼内脏废弃物为原料,通过优化磁感应电场(MIEFE)关键参数(激励电压、电场频率、流量及反应时间),建立了磁感应电场-酶解耦合提取技术(MIEF-EH),并系统完成产物纯化与结构表征,旨在实现牛磺酸的绿色、高效提取与纯化。研究结果表明:最优提取条件为激励电压1400 V、电场频率50 kHz、流量25 L/h、反应时间1 h;在优化条件下,MIEF-EH法提取量达到12.36 mg/g,显著(P<0.05)高于单一方法(MIEFE、微波、酶解及水热法)及其他耦合方法(微波-酶解、水热-酶解);SEM表征显示,经过处理后的鲍鱼内脏粒径显著减小,形成疏松多孔微结构,该变化与牛磺酸提取量提升相关;牛磺酸粗提物经离子交换树脂纯化,回收率92.29%、纯度96.78%;乙醇沉淀法回收率95.30%、纯度94.43%;FT-IR、MS及1H NMR联合分析证实纯化产物分子量与牛磺酸理论值高度吻合,化学结构与标准品一致;获得的牛磺酸产物各项理化指标符合GB 14759-2010要求。综上,本研究构建的MIEF-EH耦合技术为鲍鱼内脏废弃物向高纯度天然牛磺酸的绿色转化提供了可行技术路径。

     

    Abstract: This study established a novel magnetically induced electric field-enzymatic hydrolysis (MIEF-EH) coupled technology for the sustainable taurine extraction from abalone viscera byproducts. The results of the study indicated that the optimal extraction conditions were as follows: an excitation voltage of 1400 V, an electric field frequency of 50 kHz, a flow rate of 25 L/h, and a reaction time of 1 h. Under the optimal conditions, MIEF-EH achieved a taurine yield of 12.36 mg/g, significantly (P<0.05) outperforming both individual methods (such as MIEFE, microwave, enzymatic hydrolysis and hydrothermal) as well as other coupled approaches (microwave-enzymatic hydrolysis and hydrothermal-enzymatic hydrolysis). SEM revealed pronounced particle-size reduction and the formation of a loose, porous microstructure, correlating with improved taurine extraction. Subsequent purification via ion-exchange resin yielded taurine with 92.29% recovery and 96.78% purity, while ethanol precipitation achieved 95.30% recovery and 94.43% purity. The chemical structure and molecular mass of the purified taurine were confirmed through multi-technique validation (FT-IR, MS, and 1H NMR). All physicochemical properties complied with the specifications of the Chinese National Standard for Food Additive Taurine (GB 14759-2010). In summary, the MIEF-EH coupled technology developed in this study provides a feasible technological pathway for green conversion of abalone viscera waste into high-purity natural taurine.

     

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