Abstract: With the increasing incidence of eye-related diseases year by year, people's awareness of protecting eye health has gradually increased. More and more studies have shown that bioactive substances have positive effects in preventing eye-related diseases and promoting eye health. However, bioactive substances encounter many problems in practical applications such as low bioavailability, poor penetration through the corneal barrier, and insufficient targeted delivery efficiency. By constructing bioactive substance delivery systems, the bioavailability and nutritional intervention effects of bioactive substances can be effectively improved. This review systematically summarizes the research progress in recent years on enhancing the utilization of bioactive substances and intervening in eye-related diseases based on new delivery systems. Firstly, it reviews the bioactive substances that can intervene in eye-related diseases, including polyunsaturated fatty acids, vitamins, carotenoids, and flavonoids; secondly, it elaborates on the delivery systems for delivering bioactive substances, including lipid-based delivery systems, polymer-based delivery systems, and natural exosomes; finally, it emphasizes the application of bioactive substance delivery systems in eye health, including promoting the absorption of bioactive substances by tissues or cells, and targeted delivery of bioactive substances to achieve precise intervention. In summary, this review provides theoretical support and technical references for bioactive substance delivery systems in precisely intervening in eye-related diseases and developing products for promoting eye health.