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中国精品科技期刊2020
郭汝杞,朱秀清,王颖. 谷朊蛋白对绿豆蛋白凝胶特性及结构的影响J. 食品工业科技,2026,47(5):1−9. doi: 10.13386/j.issn1002-0306.2025050182.
引用本文: 郭汝杞,朱秀清,王颖. 谷朊蛋白对绿豆蛋白凝胶特性及结构的影响J. 食品工业科技,2026,47(5):1−9. doi: 10.13386/j.issn1002-0306.2025050182.
GUO Ruqi, ZHU Xiuqing, WANG Ying. Effects of Wheat Gluten on the Gel Properties and Structure of Mung Bean ProteinJ. Science and Technology of Food Industry, 2026, 47(5): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025050182.
Citation: GUO Ruqi, ZHU Xiuqing, WANG Ying. Effects of Wheat Gluten on the Gel Properties and Structure of Mung Bean ProteinJ. Science and Technology of Food Industry, 2026, 47(5): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025050182.

谷朊蛋白对绿豆蛋白凝胶特性及结构的影响

Effects of Wheat Gluten on the Gel Properties and Structure of Mung Bean Protein

  • 摘要: 本研究探讨了绿豆蛋白(mung bean protein,MBP)与谷朊蛋白(wheat gluten,WG)复合比例对其凝胶体系凝胶特性及结构的影响。通过调控两者的质量比例(MBP:WG分别为10:0、9:1、8:2、7:3、6:4、5:5),分析了其对热诱导(95 ℃,30 min)复合凝胶持水性、质构特性、流变特性、二三级结构及微观结构的影响。结果表明,与纯MBP相比,适量添加WG可显著改善复合凝胶的凝胶强度和致密性,特别是在MBP:WG为7::3时,凝胶表现出最佳的持水性(97.15%)和弹性(0.98)(P<0.05)。此配比下蛋白二级结构由α-螺旋向β-折叠结构转变。综上,合理调控MBP与WG的比例可显著改善复合蛋白凝胶体系的结构与功能特性,为植物蛋白在高质量食品结构设计中的应用提供理论基础和技术支持。

     

    Abstract: This study investigated the effect of the composite ratio of mung bean protein (MBP) and wheat gluten (WG) on the gel properties and structure of their gel system. The effects on the water-holding capacity, textural properties, rheological properties, secondary and tertiary structure, and microstructure of the heat-induced (95 ℃, 30 min) composite gels were analyzed by regulating the mass ratio of the two (MBP:WG was 10:0, 9:1, 8:2, 7:3, 6:4, and 5:5, respectively). The results showed that compared to pure MBP, the gel strength and densification of the composite gels could be significantly improved by the addition of WG in appropriate amounts, especially when the mass ratio of MBP and WG was 7:3, which showed the best water-holding capacity (97.15%) and springiness (0.98) of the gels (P<0.05). This was mainly attributed to the enhancement of disulfide bonds, while the FTIR spectral analysis showed that the protein secondary structure was transformed from α-helix to β-sheet structure under this ratio, which was conducive to the construction of a stable gel network structure. In conclusion, the rational regulation of the ratio of MBP to WG can significantly improve the structural and functional properties of the composite protein gel system, which provides the theoretical basis and technical support for the application of plant proteins in the structural design of high-quality foods.

     

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