Abstract: This study aimed to analyze the composition of white birch juice (WBJ) and to elucidate the alleviating effects of daily WBJ consumption on ethanol-induced intoxication in mice. The composition of WBJ was systematically characterized via untargeted metabolomics analysis coupled with quantitative determination. Twenty-eight ICR male mice were randomly and equally divided into 4 groups: Control (CON), WBJ, drunk (DRK) and DRK+WBJ. Mice in the WBJ and DRK+WBJ groups were administered WBJ for 30 d. Subsequently, mice in the DRK and DRK+WBJ groups received ethanol-induced intoxication. The ethanol-alleviating effect of WBJ was evaluated by measuring intoxication time and recovery time, heart rate (HR), respiratory rate (RR), blood oxygen saturation (SpO₂), serum activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and pathological changes in the liver. The ethanol-alleviating mechanisms of WBJ were investigated by measuring the activities of alcohol dehydrogenase (ADH), acetaldehyde dehydrogenase (ALDH), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPX), and the contents of malondialdehyde (MDA), reduced glutathione (rGSH), interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in liver tissue. The results showed that 1511 compounds were identified in WBJ, which were systematically categorized into 10 superclasses and 44 subclasses. Quantitative analysis of compositions showed that WBJ contained 99.1 g/100 g moisture, 27.6 mg/100 g ash, 1.07 g/100 mL soluble sugars, 0.88 g/100 mL reducing sugars, 63.0 mg/100 mL protein, 47.6 mg/100 g total lipids, 24.4 mg/100 mL polyphenols, and 35.7 mg/100 mL flavonoids. Additionally, 27 amino acids, 9 vitamins and 11 elements were identified. Compared to the DRK group, mice in the DRK+WBJ group exhibited shorter ethanol recovery time, stable post-intoxication HR, RR, and SpO₂, reduced serum ALT and AST activities (P<0.05), and attenuated central vein dilation in hepatic lobules. The mechanism may be attributed to daily WBJ consumption enhancing ADH and ALDH activities, elevating rGSH content, increasing CAT, SOD, and GPX activities, reducing MDA production, and suppressing IL-1β, IL-6, and TNF-α in the livers of ethanol-intoxicated mice. In this study, the composition of WBJ was characterized, and the mechanism of WBJ alleviating ethanol-induced intoxication was elucidated via regulating ethanol metabolism, enhancing antioxidant capacity, and attenuating inflammation, thereby providing a basis for the development of WBJ-based hangover relief products.