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中国精品科技期刊2020
张执,吴海虹,戴竹青,等. 植物乳植杆菌杏脆制备工艺优化与品质分析J. 食品工业科技,2026,47(10):1−10. doi: 10.13386/j.issn1002-0306.2025050229.
引用本文: 张执,吴海虹,戴竹青,等. 植物乳植杆菌杏脆制备工艺优化与品质分析J. 食品工业科技,2026,47(10):1−10. doi: 10.13386/j.issn1002-0306.2025050229.
ZHANG Zhi, WU Haihong, DAI Zhuqing, et al. Optimization of Preparation Technology and Quality Analysis of Lactiplantibacillus plantarum Enriched Apricot CrispsJ. Science and Technology of Food Industry, 2026, 47(10): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025050229.
Citation: ZHANG Zhi, WU Haihong, DAI Zhuqing, et al. Optimization of Preparation Technology and Quality Analysis of Lactiplantibacillus plantarum Enriched Apricot CrispsJ. Science and Technology of Food Industry, 2026, 47(10): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025050229.

植物乳植杆菌杏脆制备工艺优化与品质分析

Optimization of Preparation Technology and Quality Analysis of Lactiplantibacillus plantarum Enriched Apricot Crisps

  • 摘要: 本研究旨在建立高植物乳植杆菌富集的益生菌杏脆工艺。采用常压浸渍(Normal Pressure Impregnation, NPI)、超声浸渍(Ultrasonic Impregnation, UP)和真空浸渍(Vacuum Impregnation, VI)三种预处理方式与发酵工艺相结合方法,通过测定冻干杏脆中植物乳植杆菌活菌数比较不同浸渍效果,经单因素实验优化工艺参数后,进一步采用正交试验对超声功率、超声时间和发酵时间进行多因素优化;同时通过扫描电镜观察杏子细胞组织变化,并测定发酵后杏脆的总糖、总酸、总酚含量等指标。结果显示,UP处理后活菌数达1.0~2.0×107 C FU/g,显著优于真空、常压处理组(P<0.05)。实验确定最佳工艺条件为:超声功率125 W、浸渍时间12 min、发酵时间20 h,终产品活菌数提升至1.0~2.0×108 CFU/g。通过扫描电镜观察,超声浸渍处理后杏子细胞组织孔隙率、孔径显著增大,可有效促进菌体渗透。发酵后杏脆总糖、总酸、总酚含量指标与对照组存在显著差异(P<0.05)。本研究构建了超声-发酵协同增效的植物乳植杆菌杏脆制备技术,为开发高活性益生菌果脆提供了创新工艺方案,对提升杏子附加值和推动功能性休闲食品发展具有重要应用价值。

     

    Abstract: This study aimed to establish a process for preparing probiotic apricot crisps enriched with a high level of Lactiplantibacillus plantarum. To achieve this, three pretreatment methods, normal pressure impregnation (NPI), ultrasonic impregnation (UI), and vacuum impregnation (VI), were combined with fermentation technology. The viable count of L. plantarum in freeze-dried apricot crisps was determined to evaluate the impregnation efficiency of the different methods. Following the optimization of individual process parameters through single-factor experiments, orthogonal experiments were conducted to further optimize multiple factors, including ultrasonic power, ultrasonic time, and fermentation time. In parallel, changes in the apricot cell tissue structure were examined using scanning electron microscopy, and the total sugar, total acid, and total phenolic contents of the fermented apricot crisps were analyzed. The results indicated that UI treatment yielded a viable count of 1.0~2.0×107 CFU/g, which was significantly higher than that of the vacuum and normal pressure treatment groups (P<0.05). The optimal process conditions were determined as follows: ultrasonic power of 125 W, impregnation time of 12 min, and fermentation time of 20 h, under which the viable count of the final product was increased to 1.0~2.0×108 CFU/g. Scanning electron microscopy observations revealed that ultrasonic impregnation significantly increased the porosity and pore size of apricot cell tissues, effectively promoting bacterial penetration. After fermentation, the total sugar, total acid, and total phenol contents of the apricot crisps showed significant differences compared with the control group (P<0.05). This study developed an ultrasonic-fermentation synergistic technology for preparing L. plantarum-enriched apricot crisps, providing an innovative process for developing high-activity probiotic fruit crisps. It holds important application value for enhancing the added value of apricots and promoting the development of functional snack foods.

     

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