Abstract: In this study, the effects of different treatment times (40, 50, 60 s) on the changes of physicochemical properties and enzyme activities of postharvest fresh-cut Dendrocalamus brandisii (D. brandisii) shoots during storage were investigated using dielectric barrier discharge-cold plasma (DBD-CP). The results showed that DBD-CP improved the brightness of fresh-cut D. brandisii shoots with the highest contents of total phenols, total flavonoids, soluble proteins, and soluble sugars compared with CK group. At the 30th d of storage of CP₁, CP₂, and CP₃-treated D. brandisii shoots, peroxidase activity decreased by 11.13, 15.96, and 5.03 U/g (P<0.05), polyphenol oxidase activity decreased by 0.28, 1.21, and 0.62 U/g (P<0.05), phenylalanine deaminase activity decreased by 1.99, 7.19, and 5.81 U/g (P<0.05), 4-coumaroyl-coenzyme A ligase activity decreased by 3.17, 4.41, and 1.81 U/g (P<0.05), pectin content increased by 23.61%, 58.33%, and 44.44% (P<0.05), and cumulative synthesis of cellulose and lignin decreased by 7.81, 37.24, and 13.86 mg/g (P<0.05), 0.54, 0.68, and 0.55 mg/g (P<0.05), respectively. Principal component analysis showed that CP₂ had the smallest confidence circle, indicating that it had the best quality stability of D. brandisii shoots. This study provides some theoretical basis for the application of DBD-CP technology in the field of postharvest preservation and processing of D. brandisii shoots.