Abstract: The combination of Nisin with active substances can broaden its antibacterial spectrum and endow the system with antioxidant capacity, thereby synergistically enhancing food preservation efficacy; consequently, it has attracted much attention in the field of food safety research. In particular, the construction of composite films using Nisin-based compounds as functional components through different film-forming methods (such as coating, casting, extrusion, and electrospinning, etc.) has become a research hotspot in the field of food active packaging in recent years. This article begins with a brief introduction to Nisin and its antibacterial mechanism, followed by a comprehensive overview of the bioactive substances utilized in conjunction with Nisin and their synergistic effects. Then, the research progress of Nisin compounds active films in food preservation, specifically from the aspects of different film-forming techniques, is mainly reviewed; Furthermore, the limitations of the current research and the future development directions are summarized and discussed, with the expectation of providing a reference for the efficient application of Nisin in the field of food preservation.