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中国精品科技期刊2020
余东亮,刘云珍,黄露露,等. 河北产区马瑟兰干红葡萄酒非挥发性酚类物质组成及其挥发性风味特征研究J. 食品工业科技,2026,47(16):1−14. doi: 10.13386/j.issn1002-0306.2025070048.
引用本文: 余东亮,刘云珍,黄露露,等. 河北产区马瑟兰干红葡萄酒非挥发性酚类物质组成及其挥发性风味特征研究J. 食品工业科技,2026,47(16):1−14. doi: 10.13386/j.issn1002-0306.2025070048.
YU Dongliang, LIU Yunzhen, HUANG Lulu, et al. Composition of Non-Volatile Phenolic Compounds and Volatile Flavor Characteristics of Marselan Dry Red Wine from the Hebei RegionJ. Science and Technology of Food Industry, 2026, 47(16): 1−14. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025070048.
Citation: YU Dongliang, LIU Yunzhen, HUANG Lulu, et al. Composition of Non-Volatile Phenolic Compounds and Volatile Flavor Characteristics of Marselan Dry Red Wine from the Hebei RegionJ. Science and Technology of Food Industry, 2026, 47(16): 1−14. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025070048.

河北产区马瑟兰干红葡萄酒非挥发性酚类物质组成及其挥发性风味特征研究

Composition of Non-Volatile Phenolic Compounds and Volatile Flavor Characteristics of Marselan Dry Red Wine from the Hebei Region

  • 摘要: 为深入解析河北产区不同产地马瑟兰干红葡萄酒中非挥发性酚类物质(单体酚、花色苷)和挥发性风味物质的典型特征,以产区内昌黎、怀来不同酒庄的市售代表性马瑟兰干红葡萄酒为研究对象,采用超高效液相色谱-串联质谱(Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry,UPLC-MS/MS)和顶空固相微萃取-气相色谱质谱联用(Headspace Solid-phase Micro Extraction Mass Spectrometry coupled with Gas Chromatography Mass Spectrometry,HS-SPME-GC-MS)技术,对样品的非挥发性酚类物质(单体酚、花色苷)及挥发性风味物质进行检测。结果表明,不同酒样的单体酚类物质存在显著差异(P<0.05),含量范围为55.84~143.97 mg/L,其中没食子酸在25种单体酚中含量最高;不同酒样的花色苷含量也存在显著差异(P<0.05),含量范围为1.99~34.11 mg/L,其中锦葵色素-3-半乳糖苷在17种花色苷中含量最高;综合单体酚和花色苷的检测结果表明,茅台凤凰庄园2017马瑟兰和2018金士马瑟兰精选级酒样中酚类物质更丰富。通过层次聚类分析(Hierarchical Cluster Analysis,HCA),单体酚和花色苷均可有效将河北产区内不同酒庄马瑟兰酒样区分开来。同时,挥发性成分共鉴定出150种,其中9种香气活性值(Odor Activity Value,OAV)对风味贡献显著(OAV>1),包括异戊酸乙酯、乙酸异戊酯、辛酸乙酯等,赋予河北马瑟兰葡萄酒甜美馥郁的花香和果香的典型特征;挥发性成分轮廓图、聚类热图以及正交偏最小二乘判别分析(Orthogonal Partial Least Squares Discriminant Analysis,OPLS-DA)显示不同酒庄马瑟兰酒样风味成分特征各异。在OPLS-DA中进一步筛选出7种可区分河北产区不同酒庄马瑟兰酒样的主要风味特征标志物(Variable Importance in Projection,VIP值),其中苯乙醇、辛酸乙酯、己酸乙酯(VIP值>2.64)为关键区分因子。本研究为阐明河北产区不同酒庄马瑟兰葡萄酒的典型风格特征提供了数据支撑,也为深入解析同一产区微域风土差异对葡萄酒典型风格特征的影响建立了方法学参考。

     

    Abstract: To extensively analyze the typical characteristics of nonvolatile phenolic compounds (monomeric phenols and anthocyanins) and volatile flavor compounds in Marselan dry red wines from different subregions within the Hebei region, commercially representative Marselan wines from various wineries in Changli and Huailai were examined.Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry (UPLC-MS/MS) and Headspace Solid-Phase Micro-Extraction coupled with Gas Chromatography Mass Spectrometry (HS-SPME-GC-MS) techniques were employed to detect the non-volatile phenolic compounds (monomeric phenols, anthocyanins) and volatile flavor compounds in the samples. The results showed significant differences (P<0.05) in varieties of monomeric phenols (numbering 25) among different wine samples, with content ranging from 55.84 to 143.97 mg/L,where gallic acid had the highest content among the 25 monomeric phenolics. The content of anthocyanins (numbering 17) in different wine samples also differed significantly (P<0.05), ranging from 1.99 to 34.11 mg/L,where malvidin-3-galactoside has the highest content among the 17 anthocyanins.Comprehensive monomer phenol and anthocyanin detection revealed that samples from the 2017 Chateau Moutai Phoenix Marselan and 2018 Chateau Kings Selected Marselan wines were especially rich in nonvolatile phenolic compounds. Through hierarchical cluster analysis (HCA), monomeric phenols and anthocyanins could be used to effectively distinguish between Marselan wine samples from different wineries in Hebei province. Overall, 150 kinds of volatile components were identified. Of these, nine kinds of aroma Odor Activity Values (OAVs) contributed significantly to wine flavors (OAV>1). These included ethyl isovalerate, isoamyl acetate, and ethyl octanoate, which gave Hebei Marselan wine the typical characteristics of sweet and fragrant flowers and fruits. Volatile component profile, cluster thermogram, and orthogonal partial least squares discriminant analysis (OPLS-DA) highlighted differences in the characteristic flavor components in Marselan wines from different wineries. In OPLS-DA, seven main flavor characteristic markers (VIP values), which could be used to distinguish between Marselan wine samples from different wineries in Hebei province were further screened, among which phenylethanol, ethyl octanoate, and ethyl hexanoate (VIP values > 2.64) were the key distinguishing factors. This study provides data to clarify the typical style characteristics of Marselan wines from different wineries in Hebei province and establishes a methodological reference for analyzing the influence of micro-regional terroir differences in the same producing area on the typical style characteristics of wines in depth.

     

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