Abstract: The results indicate that menthol-acetic acid (DES-1) is the optimal eutectic solvent; the optimized extraction conditions are 47% (V/V) ethanol content, a material-to-liquid ratio of 1:45 (g/mL), and an extraction temperature of 69 ℃, under which the extraction yield of lutein can reach 644.11±1.99 μg/g. The lutein solution extracted and prepared with DES-1 exhibits scavenging rates of 77.52% and 84.58% for hydroxyl radicals and DPPH radicals, respectively, which are significantly higher than those achieved by traditional ethanol extraction methods (P<0.01). Under thermal conditions of 30 ℃ at 75% relative humidity and light conditions of 4500 Lux at 25 ℃, the lutein retention rates for the lutein-menthol cocrystal are 84.34% and 33.73%, both of which are significantly higher than those of commercially available lutein. The formed cocrystal structure can physically hinder oxygen penetration, while the hydroxyl groups at both ends of lutein can form strong hydrogen bonds with the hydroxyl groups of menthol, thereby significantly enhancing stability. Identification through LC-MS/MS revealed a total of 33 lutein-related components in the cocrystal, including lutein and lutein palmitate, with the highest content of free lutein reaching 107.93 ± 11.36 μg/g. The lutein-menthol cocrystal prepared in this study significantly enhances the stability of lutein and may provide more possibilities for the practical development and utilization of lutein.