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中国精品科技期刊2020
夏娜,张永松,张亚楠,等. L-赖氨酸与L-精氨酸对巴旦木仁蛋白乳化性能的改良及其结构的影响J. 食品工业科技,2026,47(13):1−8. doi: 10.13386/j.issn1002-0306.202508178.
引用本文: 夏娜,张永松,张亚楠,等. L-赖氨酸与L-精氨酸对巴旦木仁蛋白乳化性能的改良及其结构的影响J. 食品工业科技,2026,47(13):1−8. doi: 10.13386/j.issn1002-0306.202508178.
XIA Na, ZHANG Yongsong, ZHANG Yanan, et al. The Improvement of Emulsifying Properties of Almond Kernel Protein by L-lysine and L-arginine and its Structural EffectsJ. Science and Technology of Food Industry, 2026, 47(13): 1−8. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.202508178.
Citation: XIA Na, ZHANG Yongsong, ZHANG Yanan, et al. The Improvement of Emulsifying Properties of Almond Kernel Protein by L-lysine and L-arginine and its Structural EffectsJ. Science and Technology of Food Industry, 2026, 47(13): 1−8. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.202508178.

L-赖氨酸与L-精氨酸对巴旦木仁蛋白乳化性能的改良及其结构的影响

The Improvement of Emulsifying Properties of Almond Kernel Protein by L-lysine and L-arginine and its Structural Effects

  • 摘要: 为了改善巴旦木蛋白(Badam Kernel Protein, BKP)的水溶性和拓展其应用,本研究采用分别向巴旦木蛋白中引入L-精氨酸(L-Arginine,L-Arg)和L-赖氨酸(L-Lysine,L-Lys)制备BKP-L-Arg/L-Lys的混合乳液。利用傅里叶红外光谱、内源荧光光谱、紫外光谱等来表征混合乳液的结构特性和理化特性。结果表明,L-Arg/L-Lys主要通过提高BKP溶液的pH值来达到改善BKP水溶性的目的。相较于pH值变化处理,L-Arg/L-Lys处理能更显著地提高BKP的水溶性,L-Arg使BKP的水溶性从67.83%提高到83.57%(P<0.05);L-Lys使水溶性提高到81.77%。引入L-Arg和L-Lys也显著改善了BKP乳化活性及其稳定性,L-Arg和L-Lys分别使EAI提高约64.13 m2/g和57.24 m2/g(P<0.05),ESI提高了37.00%和29.02%(P<0.05)。L-Arg与L-Lys改性可有效克服BKP原有的水溶性限制,并赋予其更好的乳化能力。经改性的BKP因其优良的水溶性和乳化性,有望作为一种高性能的天然蛋白添加剂,应用于饮料、酱料、乳制品等乳液型食品体系中,具有广阔的应用前景。

     

    Abstract: To improve the water solubility of Badam Kernel Protein (BKP) and expand its application scope, this study prepared BKP-L-Arginine (L-Arg)/L-Lysine (L-Lys) mixed emulsions by introducing L-Arg and L-Lys into BKP, respectively. Fourier transform infrared spectroscopy, intrinsic fluorescence spectroscopy, ultraviolet spectroscopy, and other analytical techniques were employed to characterize the structural and physicochemical properties of the mixed emulsions. The results demonstrated that L-Arg/L-Lys primarily enhanced the water solubility of BKP by elevating the pH value of the BKP solution. In comparison to pH-adjustment treatment, L-Arg/L-Lys treatment exhibited a more remarkable effect in improving the water solubility of BKP. L-Arg increased the water solubility of BKP from 67.83% to 83.57% (P<0.05); L-Lys increased it to 81.77%. The incorporation of L-Arg and L-Lys also significantly improved the emulsifying activity and stability of BKP. Specifically, L-Arg and L-Lys increased the Emulsifying Activity Index (EAI) by approximately 64.13 m2/g and 57.24 m2/g, respectively (P<0.05), and the Emulsifying Stability Index (ESI) by 37.00% and 29.02%, respectively (P<0.05). Modification with L-Arg and L-Lys can effectively overcome the inherent water solubility limitation of BKP and confer better emulsifying capacity to it. Owing to its excellent water solubility and emulsifying properties, the modified BKP is anticipated to serve as a high-performance natural protein additive, finding widespread application in emulsion-type food systems such as beverages, sauces, and dairy products, thus possessing broad application prospects.

     

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