• 中国科技期刊卓越行动计划项目资助期刊
  • 中国精品科技期刊
  • 首都科技期刊卓越行动计划
  • EI
  • Scopus
  • CAB Abstracts
  • Global Health
  • 北大核心期刊
  • DOAJ
  • EBSCO
  • 中国核心学术期刊RCCSE A+
  • 中国科技核心期刊CSTPCD
  • JST China
  • FSTA
  • 中国农林核心期刊
  • 中国开放获取期刊数据库COAJ
  • CA
  • WJCI
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
中国精品科技期刊2020
陈强强,寇肖迪,徐海俊,等. 蜡样芽孢杆菌在三种不同乳产品中的生长动力学特性以及引起乳制品感官变化特征J. 食品工业科技,2026,47(17):1−11. doi: 10.13386/j.issn1002-0306.2025090242.
引用本文: 陈强强,寇肖迪,徐海俊,等. 蜡样芽孢杆菌在三种不同乳产品中的生长动力学特性以及引起乳制品感官变化特征J. 食品工业科技,2026,47(17):1−11. doi: 10.13386/j.issn1002-0306.2025090242.
CHEN Qiangqiang, KOU Xiaodi, XU Haijun, et al. Growth Kinetics and Organoleptic Characterization of Bacillus cereus in Three Different Dairy ProductsJ. Science and Technology of Food Industry, 2026, 47(17): 1−11. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025090242.
Citation: CHEN Qiangqiang, KOU Xiaodi, XU Haijun, et al. Growth Kinetics and Organoleptic Characterization of Bacillus cereus in Three Different Dairy ProductsJ. Science and Technology of Food Industry, 2026, 47(17): 1−11. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025090242.

蜡样芽孢杆菌在三种不同乳产品中的生长动力学特性以及引起乳制品感官变化特征

Growth Kinetics and Organoleptic Characterization of Bacillus cereus in Three Different Dairy Products

  • 摘要: 为探究蜡样芽孢杆菌在不同牛奶产品中的生长动力学特性及对牛奶品质影响,本研究采集了50份乳制品(包括25份工厂前处理罐原料乳和25份市售纸盒包装商品乳),通过甘露醇卵黄多粘菌素琼脂培养基(MYP)分离疑似菌株,结合形态学观察、蛋白质毒素结晶实验、Vitek-2生化鉴定及16S rRNA测序分离鉴定蜡样芽孢杆菌;以筛选生长速率最快的菌株作为实验对象,按101、102、103 CFU/mL初始浓度回接至三种不同牛奶产品(纯牛奶、舒化牛奶和谷粒牛奶)中,研究在两种温度(25 ℃和35 ℃)下,蜡样芽孢杆菌在三种不同产品中的生长动力学,采用Logistic方程拟合生长模型,同步监测三种牛奶产品的pH变化和感官影响。结果显示:分离鉴定的3株蜡样芽孢杆菌(B1、B2和B3)均源自原料乳,市售乳中未检出;通过生长曲线测定,研究B1菌株对三种不同牛奶产品的影响,结果表明温度和初始接种浓度对蜡样芽孢杆菌的生长影响较为明显,在35 ℃条件下和高初始接种浓度下,乳制品中的蜡样芽孢杆菌较25 ℃更早进入对数期和稳定期,迟滞期更短,三种类型的产品中,舒化牛奶更有利于蜡样芽孢杆菌的生长;Logistic模型拟合度R2>0.9857;牛奶的pH随菌浓度增长变化明显,不同乳制品感官劣变具有各自的特异性,其中舒化牛奶气味劣变最为显著。本研究为乳制品蜡样芽孢杆菌污染预警及安全控制提供科学依据

     

    Abstract: The purpose of this study was to investigate the growth kinetics of Bacillus cereus in different milk products and its impact on milk quality. Fifty dairy products (including 25 raw milk from factory pre-treatment tanks and 25 commercial milk packaged in boxes) were collected, and suspected strains were isolated using Mannitol-Egg-Yolk-Polymyxin Agar (MYP). Morphological observation, protein toxin crystallization experiment, Vitek-2 biochemical identification, and 16S rRNA sequencing were used to isolate and identify B. cereus. Further, one strain with the fastest growth rate was selected as the experimental object, and the strain was inoculated into three different milk products (pure milk, lactose-decomposed milk, and grain milk) at initial concentrations of 101, 102, and 103 CFU/mL. The growth kinetics of B. cereus in three different products were studied at two temperatures (25 ℃ and 35 ℃), and a Logistic equation was used to fit the growth model. The pH and sensory changes of the three milk products were simultaneously monitored. The results showed that the three strains (B1, B2, and B3) of B. cereus originated from raw milk were isolated and identified and they were not detected in commercial milk products. By measuring the growth curve, B1 strain with a faster growth rate was selected as the experimental object to study its effects on three different milk products. The results showed that temperature and initial inoculation concentration had a more significant impact on the growth of B. cereus. At 35 ℃ and high initial inoculation concentration, B. cereus in dairy products entered the logarithmic and stable phases earlier than at 25 ℃, with a shorter lag period. Among the three types of products, lactose-decomposed milk was more conducive to the growth of B. cereus. The fitting degree of the Logistic model was R2>0.9857. It was found that the pH of milk changed significantly as the bacterial concentration increased. Different dairy products had their own characteristics in terms of sensory degradation. Among them, the odor deterioration of lactose-decomposed milk was the most significant. This study provides a scientific basis for the early warning and safety control of B. cereus contamination in dairy products.

     

/

返回文章
返回